Anti-Human Nuclei (OAPC00049)

Catalog No: OAPC00049

Size:100 ul

Immunostaining of HeLa cells showing specific labeling of their nuclei using our anti-nuclei antibody.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid100 ul culture supernatant. Adequate amount of material to conduct 10-mini Western blots
• Clonality: Monoclonal
• Clone: 235-1
• Isotype: IgG₁
• Host: Mouse
• Application: IHC, IF
• Reconstitution and Storage: Store at -20C in undiluted aliquots; stable for up to one year after date of receipt. Avoid freeze/thaw cycles.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 1 mg/ml
• Specificity: Specific for human nuclei. Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells.
  By immunoprecipitation the antibody reacts with an 80 kDa and 70 kDa band. The antibody works on acetone fixed and paraformaldehyde fixed cells. It is suggested that you permeabilize the cells with 0.1-0.4% Triton X100. The antibody also works for immunohistochemistry on paraformaldehyde fixed cryostat sections.
• Application Info: IF/IHC: 1:100
• Reference: Glaser R, Lu MM, Narula N, Epstein JA (2002) Smooth muscle cells, but not myocytes, of host origin in transplanted human hearts. Circulation Jul 2;106(1):17-9.
• Quality Control: Immunofluorescence performed on each lot

Target Info

• Description of Target: This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells, with no reactivity to rat or mouse cells.
• Molecular Weight: 80kDa & 70kDa