- Description of Target:
- The function of this protein remains unknown.
- Gene Symbol:
- Official Gene Full Name:
- Activating transcription factor 4
- NCBI Gene Id:
- Alias Symbols:
- Atf-4; C/ATF; CREB2; MGC96460; TAXREB67
- Tissue Tool:
- Find tissues and cell lines supported to express Atf4.
- Protein Accession #:
- Nucleotide Accession#:
- Swissprot Id:
- Protein Name:
- Cyclic AMP-dependent transcription factor ATF-4
- Protein Size (# AA):
- Molecular Weight:
- IF, WB
- Protein Interactions:
- Egln3; Ubc; Hif1a; Satb2; Fam175b; Trib3; Gabbr2; Gabbr1; Jun; Fos; Dapk3; Rps6ka3; Tnfsf11; Prkaca;
- The immunogen for anti-Atf4 antibody: synthetic peptide corresponding to a region of Mouse
- Product Format:
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
- Affinity Purified
- Complete computational species homology data:
- Atf4 Antibody (ARP37018_P050)
- Predicted Homology Based on Immunogen Sequence:
- Pig: 100%; Rat: 100%; Mouse: 100%; Sheep: 100%; Bovine: 100%; Guinea pig: 100%; Dog: 93%; Human: 93%; Horse: 79%
- Species Reactivity:
- Pig, Rat, Sheep, Bovine, Guinea pig, Mouse, Human, Dog, Horse
- Datasheets / Downloads:
- Printable datasheet for
anti-Atf4 antibody- Printable datasheet for
anti-Atf4 antibody- ARP37018_P050
- Peptide Sequence:
- Synthetic peptide located within the following region: TRYRQKKRAEQEALTGECKELEKKNEALKEKADSLAKEIQYLKDLIEEVR
- Blocking Peptide:
- For anti-Atf4 antibody is For anti-Atf4 antibody is Catalog # AAP37018 (Previous Catalog # AAPP09927)
- Reconstitution and Storage:
- For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Customer Reviews for Atf4 Antibody (ARP37018_P050) tested with mouse corti organ in Immunofluorescence
Drs. Gilda Kalinec and Pru Thein
CB&G House Research Institute
Catalog Number: ARP37018_P050
1: Halterman MW, Gill M, DeJesus C, Ogihara M, Schor NF, Federoff HJ.The endoplasmic reticulum stress response factor CHOP-10 protects against hypoxia-induced neuronal death.J Biol Chem.2010 Jul 9;285(28):21329-40.Epub 2010 May 6.PubMed PMID: 20448044; PubMed Central PMCID: PMC2898390.
Product Name: Atf4 Antibody (ARP37018_P050)
Gene Name: Atf4
Sample description: Transcriptional activator.Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters.It binds to a Tax-responsive enhancer element in the long terminal repeat of HTLV-I.
Experiment Name: Western Blotting
Whole cell lysates were harvested from 2.5 x106 neurons cultured in 60-mm dishes by first rinsing once with ice-cold PBS and then harvesting after incubation on ice for 10 min with RIPA buffer (Tris-HCl (50mM, pH 7.4), 1% Nonidet P-40, 0.25% sodium deoxycholate (150 mM NaCl), 1 mM EDTA, 1 protease inhibitor mixture).Samples were centrifuged at 12,000 rpm for 15 min at 4 C in a bench top centrifuge, and supernatants were stored at -70 C.Aliquots of equal volume were resolved by SDS-PAGE (8-15%), transferred to polyvinylidene difluoride membranes (Millipore, Bedford, MA) and blocked for 90 min at room temperature in wash buffer (50 mM Tris, 0.9% NaCl, 0.05% Tween 20) containing 5% nonfat dry milk.Primary antibodies were added with constant agitation overnight at 4 C, blots were rinsed thee times in wash buffer, and incubated with horseradish peroxidase-conjugated secondary antibody for 1 h (diluted 1:2000, Santa Cruz Biotechnology), washed, and detected by ECL (Renaissance, Amersham Biosciences, Piscataway, NJ).The following antibodies were used: NeuN (Chemicon, Temecula, CA); cleaved caspase-3 and PARP, eIF2alpha , and p-eIF2 ser51 were obtained from Cell Signaling Technologies (Beverly, MA); ATF4 (AVIVA, San Diego, CA); Tribbles 3 (A-20 and T-15) and CHOP-10 (B-3 and F-168) antibodies were from Santa Cruz Biotechnology.Actin (A4700) was from Sigma-Aldrich.
(i) In the current study Marc et al.used expression microarrays to study the genetic mechanisms regulating this transition from adaptive to pathological transition in hypoxic dissociated neuronal In addition to characterizing the temporal sequence of hypoxia-induced transcriptional responses, they discovered that, in contrast to ATF4, native expression of the related bZIP heterodimeric factor CHOP-10 was not predictive of in vitro neuron loss as expected from prior published observations.cultures..
(ii) Given the importance of CHOP-10 in ER-mediated responses after stroke, they analyzed the temporal activation of factors involved in CHOP signaling by Western blotting.As expected, hypoxia induced both the early and reversible phosphorylation of the elongation factor eIF2alpha in vitro (3-6 h), as well as the induction of the CHOP heterodimeric partner ATF4, due likely to cap-independent, internal ribosome entry site-mediated expression of latent ATF4 transcripts.
Product page for Atf4 antibody (ARP37018)
The information below lists the predicted species and target name associated to the peptide antigen. Please note, all available target reference numbers to the antigen sequence are presented, including unreviewed and protein isoforms.
To search for antibodies by species, please visit Aviva’s Species Reactivity Page. To search Aviva’s catalog of antibodies by sequence, please visit Aviva’s Antibody Blast Tool.
|Predicted Species & Target||Target Reference||Predicted Homology|
|African elephant ATF4 antibody; Loxodonta africana ATF4 antibody||G3T8L6||92%|
|Bovine ATF4 antibody; Bos taurus ATF4 antibody||Q3ZCH6||100%|
|Bovine Bt.76048 antibody; Bos taurus Bt.76048 antibody||F1MXZ1||100%|
|Chicken ATF4 antibody; Gallus gallus ATF4 antibody||Q9W610||85%|
|Chinese hamster Atf4 antibody; Cricetulus griseus Atf4 antibody||A4PIT2||100%|
|Common turkey ATF4 antibody; Meleagris gallopavo ATF4 antibody||G1NIF6||85%|
|Dog ATF4 antibody; Canis familiaris ATF4 antibody||F1PLQ2||92%|
|Duckbill platypus ATF4 antibody; Ornithorhynchus anatinus ATF4 antibody||F7CWP7||85%|
|Giant panda LOC100476284 antibody; Ailuropoda melanoleuca LOC100476284 antibody||D2GZL3||92%|
|Gray short-tailed opossum ATF4 antibody; Monodelphis domestica ATF4 antibody||F7CPU4||85%|
|Green anole ATF4 antibody; Anolis carolinensis ATF4 antibody||G1KGS8||78%|
|Guinea pig ATF4 antibody; Cavia porcellus ATF4 antibody||H0VUY0||100%|
|Horse LOC100070334 antibody; Equus caballus LOC100070334 antibody||F6V5P0||78%|
|Human ATF4 antibody; Homo sapiens ATF4 antibody||P18848||92%|
|Human ATF4 antibody; Homo sapiens ATF4 antibody||Q96AQ3||92%|
|Human ATF4 antibody; Homo sapiens ATF4 antibody||Q6ICP0||92%|
|Lowland gorilla ATF4 antibody; Gorilla gorilla gorilla ATF4 antibody||G3R3N8||92%|
|Mouse ATF4 antibody; Mus musculus ATF4 antibody||Q06507||100%|
|Mouse Atf4 antibody; Mus musculus Atf4 antibody||Q8CF69||100%|
|Mouse Atf4 antibody; Mus musculus Atf4 antibody||Q794G7||100%|
|Mouse Atf4 antibody; Mus musculus Atf4 antibody||Q61328||100%|
|Mouse Atf4 antibody; Mus musculus Atf4 antibody||E9Q729||100%|
|Mouse Atf4 antibody; Mus musculus Atf4 antibody||Q3U2J1||92%|
|Northern white-cheeked gibbon ATF4 antibody; Nomascus leucogenys ATF4 antibody||G1RZ45||92%|
|Pig ATF4 antibody; Sus scrofa ATF4 antibody||A0N0D5||100%|
|Pig CREB2 antibody; Sus scrofa CREB2 antibody||B2LVG7||100%|
|Pig Ssc.37849 antibody; Sus scrofa Ssc.37849 antibody||E3VJQ3||100%|
|Rat ATF4 antibody; Rattus norvegicus ATF4 antibody||Q9ES19||100%|
|Rat Atf4 antibody; Rattus norvegicus Atf4 antibody||F8WFL8||100%|
|Rat Atf4 antibody; Rattus norvegicus Atf4 antibody||B0BMW3||100%|
|Rhesus macaque ATF4 antibody; Macaca mulatta ATF4 antibody||F7GFP2||92%|
|Sheep ATF4 antibody; Ovis aries ATF4 antibody||B6Z9S5||100%|
|Small-eared galago ATF4 antibody; Otolemur garnettii ATF4 antibody||H0XLZ1||100%|
|Tasmanian devil ATF4 antibody; Sarcophilus harrisii ATF4 antibody||G3WM84||85%|
|Zebra finch ATF4 antibody; Taeniopygia guttata ATF4 antibody||H0ZIX8||85%|
Aviva Systems Biology is the original manufacturer of this Atf4 antibody (ARP37018_P050)
Click here to view the Atf4 antibody Western Blot Protocol
Product Datasheet Link: Atf4 antibody (ARP37018_P050)
WB Suggested Anti-Atf4 Antibody Titration: 0.2-1 ug/ml
Positive Control: Mouse Brain
Western Blot image:
Description of Target: The function of this protein remains unknown.
Questions pertaining to this data can be directed to firstname.lastname@example.org
Aviva Systems Biology’s Atf4 antibody (ARP37018_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at email@example.com.
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