Catalog No: OKEH04655
Size:96T
Price: $525.00
SKU
OKEH04655
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Rat, Rattus norvegicus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Psmb9 ELISA Kit (Rat) (OKEH04655) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Psmb9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Psmb9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Psmb9 captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/ml | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-Assay CV: < 10% Mean Inter-Assay CV: < 12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 0.166 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Rat Proteasome subunit beta type-9 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | Psmb9 |
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Gene Full Name | proteasome 20S subunit beta 9 |
Alias Symbols | large multifunctional protease 2;Lmp2;low molecular mass polypeptide 2;low molecular mass protein 2;macropain chain 7;multicatalytic endopeptidase complex chain 7;proteasome (prosome, macropain) subunit, beta type 9 (large multifunctional peptidase 2);proteasome (prosome, macropain) subunit, beta type, 9;proteasome chain 7;proteasome subunit beta 9;proteasome subunit beta type-9;proteasome subunit beta-1i;proteosome (prosome, macropain) subunit, beta type 9;really interesting new gene 12 protein;RING12 protein. |
NCBI Gene Id | 24967 |
Protein Name | P28077 |
Description of Target | Proteasome subunit beta type-9 |
Uniprot ID | https://www.uniprot.org/uniprot/P28077 |
Protein Accession # | NP_036840.2 |
Nucleotide Accession # | NM_012708.2 |
Protein Size (# AA) | 219 |
Molecular Weight | 23 KDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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