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Catalog No: OKEH04654
Size:96T
Price: $525.00
SKU
OKEH04654
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Psmb9 ELISA Kit (Mouse) (OKEH04654) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Psmb9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Psmb9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Psmb9 captured in well.
ELISA Kit Range0.312-20ng/ml
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-Psmb9 Microplate96 Wells (12 x 8 Well Strips)
Psmb9 Lyophilized Standard2
Sample Diluent1 x 20 mL
Psmb9 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.165 ng/mL
SpecificityNatural and recombinant Mouse Proteasome subunit beta type-9
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPsmb9
Gene Full Nameproteasome (prosome, macropain) subunit, beta type 9 (large multifunctional peptidase 2)
Alias Symbolslarge multifunctional protease 2;Lm;Lmp;Lmp2;Lmp-2;LMP-2d;low molecular mass protein 2;macropain chain 7;multicatalytic endopeptidase complex chain 7;proteasome chain 7;proteasome subunit beta type-9;proteasome subunit beta-1i;proteosome (prosome, macropain) subunit, beta type 9 (large multifunctional peptidase 2);proteosome beta 9 subunit;really interesting new gene 12 protein.
NCBI Gene Id16912
Protein NameP28076
Description of TargetProteasome subunit beta type-9
Uniprot IDhttps://www.uniprot.org/uniprot/P28076
Protein Accession #NP_038613.1
Nucleotide Accession #NM_013585.2
Protein Size (# AA)219
Molecular Weight23 KDa
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