Psmb9 ELISA Kit (Rat) (OKEH04655)

Catalog No: OKEH04655

Size:96T

Rat Psmb9 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Psmb9 ELISA Kit (Rat) (OKEH04655) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Psmb9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Psmb9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Psmb9 captured in well.
• ELISA Kit Range: 0.312-20ng/ml
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • Anti-Psmb9 Microplate: 96 Wells (12 x 8 Well Strips)
  • Psmb9 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • Psmb9 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.166 ng/mL
• Specificity: Natural and recombinant Rat Proteasome subunit beta type-9
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Psmb9
• Gene Full Name: proteasome 20S subunit beta 9
• Alias Symbols: large multifunctional protease 2;Lmp2;low molecular mass polypeptide 2;low molecular mass protein 2;macropain chain 7;multicatalytic endopeptidase complex chain 7;proteasome (prosome, macropain) subunit, beta type 9 (large multifunctional peptidase 2);proteasome (prosome, macropain) subunit, beta type, 9;proteasome chain 7;proteasome subunit beta 9;proteasome subunit beta type-9;proteasome subunit beta-1i;proteosome (prosome, macropain) subunit, beta type 9;really interesting new gene 12 protein;RING12 protein.
• NCBI Gene Id: 24967
• Protein Name: P28077
• Description of Target: Proteasome subunit beta type-9
• Uniprot ID: https://www.uniprot.org/uniprot/P28077
• Protein Accession #: NP_036840.2
• Nucleotide Accession #: NM_012708.2
• Protein Size (# AA): 219
• Molecular Weight: 23 KDa