MET Antibody (Phospho-Y1234/Y1235) (OAAB06602)

Catalog No: OAAB06602

Size:400ul

Detection of endogenous Met in HepG2 cell line. 10 ug/lane of HepG2 cell lysate was used to examine the expression of human Met. Lanes 1-5 represent Aviva's different anti-Met monoclonal antibodies. Lane 6 represents auto-phosohorylated-Met in HepG2 cell line detected by anti-phospho-Met Mab.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Monoclonal
• Clone: 6AT1877
• Isotype: IgG1
• Host: Mouse
• Application: WB
• Additional Information: Function: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. May regulate cortical bone osteogenesis.
  Cellular Location: Membrane; Single-pass type I membrane protein
  Tissue Location Expressed in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine. Found also in basal keratinocytes of esophagus and skin. High levels are found in liver, gastrointestinal tract, thyroid and kidney. Also present in the brain. Expressed in metaphyseal bone (at protein level).
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This monoclonal antibody is generated from mice immunized with a KLH conjugated synthetic peptide corresponding to sequences in Tyr1234/1235 region of human Met and residues both Tyr1234 and Tyr1235 are phosphorylated.
• Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Concentration: 0.45 mg/ml
• Application Info: WB: 1:100~500
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.

Target Info

• Gene Symbol: MET
• Alias Symbols: HGFR, AUTS9, RCCP2, c-Met, DFNB97
• NCBI Gene Id: 4233
• Protein Name: Hepatocyte growth factor receptor
• Description of Target: The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: P08581
• Protein Accession #: NP_000236.2
• Molecular Weight: 156 kDa