MET/HGFR Antibody (OAAB06603)

Catalog No: OAAB06603

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Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with MET/HGFR Antibody (Cat.#OAAB06603), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Clonality: Monoclonal
• Clone: 4AT44
• Isotype: IgG1
• Host: Mouse
• Application: WB, IHC, IF
• Additional Information: Function: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1 Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells
  Cellular Location: Membrane; Single-pass type I membrane protein
  Tissue Location: Expressed in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine. Found also in basal keratinocytes of esophagus and skin. High levels are found in liver, gastrointestinal tract, thyroid and kidney. Also present in the brain
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This monoclonal antibody is generated from mice immunized with purified recombinant protein encoding the catalytic domain of human Met.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: IHC-P: 1:25
  WB: 1:1000
  IF: 1:100
  WB: 1:100~500
  IHC-P: 1:50~100
• Intended Use: MET/HGFR Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Target Info

• Gene Symbol: MET
• Alias Symbols: HGFR, AUTS9, RCCP2, c-Met, DFNB97
• NCBI Gene Id: 4233
• Protein Name: Hepatocyte growth factor receptor
• Description of Target: Purified Mouse Monoclonal Antibody (Mab)
• Uniprot ID: P08581
• Protein Accession #: NP_000236.2
• Molecular Weight: 155541 Da