MET Antibody (OAAF01005)

Catalog No: OAAF01005

Size:100 ug

Western blot analysis of extracts from HepG2 cells, using c-Met Antibody. The lane on the right is treated with the synthesized peptide.

Immunofluorescence analysis of A549 cells, using c-Met Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human MET.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
• Specificity: c-Met antibody detects endogenous levels of total c-Met protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:1000; IF: 1:100~1:500; ELISA: 1:1000
• Reference: Hillier L.W., Nature 424:157-164(2003).
  Wang D., J. Biol. Chem. 277:36216-36222(2002).
  Giordano S., Nat. Cell Biol. 4:720-724(2002)

Target Info

• Gene Symbol: MET
• Gene Full Name: MET proto-oncogene, receptor tyrosine kinase
• Alias Symbols: AUTS9;c-Met;DFNB97;hepatocyte growth factor receptor;HGF receptor;HGF/SF receptor;HGFR;proto-oncogene c-Met;RCCP2;scatter factor receptor;SF receptor;tyrosine-protein kinase Met.
• NCBI Gene Id: 4233
• Protein Name: Hepatocyte growth factor receptor
• Description of Target: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. May regulate cortical bone osteogenesis (By similarity).
• Uniprot ID: P08581
• Protein Accession #: NP_000236.2
• Molecular Weight: 155 kDa