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| Datasheets/Manuals | Printable datasheet for CK7 Antibody (OAAD00139) - OAAD00139 |
|---|
| Predicted Species Reactivity | Human |
|---|---|
| Clonality | Monoclonal |
| Clone | CK-7 |
| Isotype | IgG1 |
| Host | Mouse |
| Application | WB, IHC, IP |
| Reconstitution and Storage | At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for longer time. |
| Immunogen | Cytoskeletal preparation of the RT4human bladder carcinoma cell line. |
| Purification | Purified by the goat anti-mouse IgG Affinity chromatography. |
| Specificity | Human No cross reactivity with other proteins. |
| Formulation | Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.01mg NaN3 as preservative. |
| Application Info | Western blot At 1-2ug/ml with the appropriate system to detect cytokeratin peptide 7 in cells and tissues. Immunohistochemistry(P) At 2-4ug/ml to detect cytokeratin peptide 7 in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) At 2-4ug/ml to detect cytokeratin peptide 7 in formalin or acetone fixed tissues. Other applications have not been tested. Optimal dilutions should be determined by end user. |
| Storage Buffer | 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml. |
| Reference | 1. Rosenberg, M.; Fuchs, E.; Le Beau, M. M.; Eddy, R. L.; Shows, T. B. : Three epidermal and one simple epithelial type II keratin genes map to human chromosome 12. Cytogenet. Cell Genet. 57: 33-38, 1991. 2. Smith, F. J. D.; Porter, R. M.; Corden, L. D.; Lunny, D. P.; Lane, E. B.; McLean, W. H. I. : Cloning of human, murine, and marsupial keratin 7 and a survey of K7 expression in the mouse. Biochem. Biophys. Res. Commun. 297: 818-827, 2002. |
| Gene Symbol | KRT7 |
|---|---|
| Alias Symbols | K7, CK7, SCL, K2C7 |
| NCBI Gene Id | 3855 |
| Protein Name | Keratin, type II cytoskeletal 7 |
| Description of Target | KRT7 is a type II keratin of simple nonkeratinizing epithelia. The deduced 489-amino acid protein has a calculated molecular mass of about 54 kD. K7 contains 4 central alpha-helical segments with heptad repeats of hydrophobic residues characteristic of a coiled-coil region. Within this domain, K7 shares 73% homology with epidermal K6B. KRT7 gene contains 9 exons and spans more than 15.6 kb. K7 gene is mapped to chromosome 12. Keratin 7 is expressed in a wide range of epithelial structures in humans. |
| Uniprot ID | P08729 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "CK7 Antibody (OAAD00139)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "CK7 Antibody (OAAD00139)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "CK7 Antibody (OAAD00139)" provided in?
This item is provided in "".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "CK7 Antibody (OAAD00139)"?
This target may also be called "K7, CK7, SCL, K2C7" in publications.
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What is the shipping cost for "CK7 Antibody (OAAD00139)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "CK7 Antibody (OAAD00139)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "CK7 Antibody (OAAD00139)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "CK7 Antibody (OAAD00139)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "KRT7"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "KRT7"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "KRT7"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "KRT7"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "KRT7"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "KRT7"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
