KRT7 ELISA Kit (Human) (OKCD01228)

Catalog No: OKCD01228 (Formerly GWB-KBBNI3)

Size:96WELLS

Human KRT7 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	89-96%	82-101%	98-105%	99-105%
  EDTA plasma(n=5)	82-96%	80-101%	92-101%	93-101%
  heparin plasma(n=5)	93-105%	78-101%	89-96%	90-97%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytokeratin 7 (CK7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytokeratin 7 (CK7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytokeratin 7 (CK7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cytokeratin 7 (CK7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	82-101	97
  EDTA plasma(n=5)	80-98	86
  heparin plasma(n=5)	89-101	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytokeratin 7 (CK7) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytokeratin 7 (CK7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-KRT7 Microplate: 96 Wells (12 x 8 Well Strips)
  • KRT7 Lyophilized Standard: 2 x 5 ng
  • 100X Biotinylated KRT7 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 28 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Keratin 7 (KRT7). No significant cross-reactivity or interference between Keratin 7 (KRT7) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: KRT7
• Gene Full Name: keratin 7
• Alias Symbols: CK7;CK-7;cytokeratin 7;Cytokeratin-7;K2C7;K7;keratin 7, type II;keratin, 55K type II cytoskeletal;keratin, simple epithelial type I, K7;keratin, type II cytoskeletal 7;Keratin-7;sarcolectin;SCL;type II mesothelial keratin K7;type-II keratin Kb7.
• NCBI Gene Id: 3855
• Protein Name: Keratin, type II cytoskeletal 7
• Description of Target: Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
• Uniprot ID: P08729
• Protein Accession #: NP_005547
• Nucleotide Accession #: NM_005556.3
• Protein Size (# AA): 469