CD9 ELISA Kit (Human) (OKCD00751)

Catalog No: OKCD00751 (Formerly GWB-KBBLZ3)

Size:96WELLS

Human CD9 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	84-95%	98-105%	90-101%	97-104%
  EDTA plasma(n=5)	78-96%	88-95%	94-101%	86-98%
  heparin plasma(n=5)	92-104%	79-102%	78-104%	78-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Motility Related Protein (MRP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Motility Related Protein (MRP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Motility Related Protein (MRP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Motility Related Protein (MRP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.2-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	83-90	86
  EDTA plasma(n=5)	96-104	101
  heparin plasma(n=5)	97-105	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Motility Related Protein (MRP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Motility Related Protein (MRP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CD9 Microplate: 96 Wells (12 x 8 Well Strips)
  • CD9 Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated CD9 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 12.5 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Motility Related Protein (MRP1). No significant cross-reactivity or interference between Motility Related Protein (MRP1) and analogues was observed.
• Publications:

  ALPPL2 Is a Potential Diagnostic Biomarker for Pancreatic Cancer-Derived Extracellular Vesicles. Mol Ther Methods Clin Dev. 15, 204-210 (2019). 31687420

• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Description:
  View product citations for MRP1 ELISA kit (Human) on CiteAb

Target Info

• Gene Symbol: CD9
• Gene Full Name: CD9 molecule
• Alias Symbols: 5H9 antigen;antigen CD9;BA-2/p24 antigen;BTCC-1;CD9 antigen;CD9 antigen (p24);cell growth-inhibiting gene 2 protein;DRAP-27;leukocyte antigen MIC3;MIC3;motility related protein-1;Motility-related protein;MRP-1;p24;tetraspanin-29;TSPAN29;TSPAN-29.
• NCBI Gene Id: 928
• Protein Name: CD9 antigen
• Description of Target: Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
• Uniprot ID: P21926
• Protein Accession #: NP_001760
• Nucleotide Accession #: NM_001769.3
• Protein Size (# AA): 228