CD9 Antibody - N-terminal region (OAAB01236)

Catalog No: OAAB01236

Size:400ul

Western blot analysis of CD9 Antibody (N-term) in Jurkat cell lysates (35ug/lane). CD9 (arrow) was detected using the purified Pab (1:60 dilution).

Western blot analysis of CD9(arrow) using rabbit polyclonal CD9 Antibody (N-term) either nontransfected (Lane 1) or lysates transiently transfected transfected with the CD9 gene (Lane 2) (Origene Technologies).

Flow cytometric analysis of jurkat cells using CD9 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, FC
  Function: Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
  Cellular Location: Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
  Tissue Location: Detected in platelets (at protein level). Expressed by a variety of hematopoietic and epithelial cells.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This CD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-51 amino acids of human CD9.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: 2 mg/ml
• Application Info: WB: 1:1000
  FC: 1:10~50
• Other Applications Image 1 Data: Flow cytometric analysis of jurkat cells using CD9 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: CD9
• Alias Symbols: MIC3, MRP-1, BTCC-1, DRAP-27, TSPAN29, TSPAN-29
• NCBI Gene Id: 928
• Protein Name: CD9 antigen
• Description of Target: CD9 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. In addition, the protein appears to promote muscle cell fusion and support myotube maintenance.
• Uniprot ID: P21926
• Protein Accession #: NP_001760.1
• Molecular Weight: 25 kDa