Catalog No: OKCD00751 (Formerly GWB-KBBLZ3)
Size:96 Wells
Price: $650.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
EDTA plasma(n=5)78-96%88-95%94-101%86-98%
heparin plasma(n=5)92-104%79-102%78-104%78-105%
ELISA Kit PrincipleAviva Systems Biology CD9 ELISA Kit (Human) (OKCD00751) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CD9captured in well.
ELISA Kit Range31.25-2,000 pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
EDTA plasma(n=5)96-104101
heparin plasma(n=5)97-105101
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level CD9 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level CD9 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
ELISA Kit Component
Anti-CD9 Microplate96 Wells (12 x 8 Well Strips)
CD9 Lyophilized Standard2 x 2 ng
100X Biotinylated CD9 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity< 12.5 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Motility Related Protein (MRP1). No significant cross-reactivity or interference between Motility Related Protein (MRP1) and analogues was observed.

ALPPL2 Is a Potential Diagnostic Biomarker for Pancreatic Cancer-Derived Extracellular Vesicles. Mol Ther Methods Clin Dev. 15, 204-210 (2019). 31687420

Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCD9
Gene Full NameCD9 molecule
Alias SymbolsMIC3, MRP-1, BTCC-1, DRAP-27, TSPAN29, TSPAN-29
NCBI Gene Id928
Protein NameCD9 antigen
Description of TargetInvolved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.3 Publications

Manually curated information for which there is published experimental evidence.


Manual assertion based on experiment iniRef.11"Suppression of cell motility and metastasis by transfection with human motility-related protein (MRP-1/CD9) DNA." Ikeyama S., Koyama M., Yamaoko M., Sasada R., Miyake M. J. Exp. Med. 177:1231-1237(1993) [PubMed] [Europe PMC] [Abstract]Cited for: ROLE IN CELL MOTILITY AND METASTASIS.Ref.12"CD9-regulated adhesion. Anti-CD9 monoclonal antibody induce pre-B cell adhesion to bone marrow fibroblasts through de novo recognition of fibronectin." Masellis-Smith A., Shaw A.R. J. Immunol. 152:2768-2777(1994) [PubMed] [Europe PMC] [Abstract]Cited for: ROLE IN CELL ADHESION.Ref.13"Structural requirements for the inhibitory action of the CD9 large extracellular domain in sperm/oocyte binding and fusion." Higginbottom A., Takahashi Y., Bolling L., Coonrod S.A., White J.M., Partridge L.J., Monk P.N. Biochem. Biophys. Res. Commun. 311:208-214(2003) [PubMed] [Europe PMC] [Abstract]Cited for: ROLE IN SPERM-EGG FUSION.
Uniprot IDP21926
Protein Accession #NP_001760
Nucleotide Accession #NM_001769.3
Protein Size (# AA)228
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