SSB ELISA Kit (Mouse) (OKCD02908)

Catalog No: OKCD02908

Size:96WELLS

Mouse SSB ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sjogren Syndrome Antigen B (SSB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sjogren Syndrome Antigen B (SSB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sjogren Syndrome Antigen B (SSB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Sjogren Syndrome Antigen B (SSB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sjogren Syndrome Antigen B (SSB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sjogren Syndrome Antigen B (SSB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SSB Microplate: 96 Wells (12 x 8 Well strips)
  • SSB Lyophilized Standard: 2
  • 100X Biotinylated SSB Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 0.058 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sjogren Syndrome Antigen B (SSB). No significant cross-reactivity or interference between Sjogren Syndrome Antigen B (SSB) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Ssb
• Gene Full Name: Sjogren syndrome antigen B
• Alias Symbols: autoantigen La;la autoantigen homolog;La protein;la ribonucleoprotein;lupus La protein homolog;Lupus LA protein homolog (LA ribonucleoprotein) (LA autoantigen homolog);SS;SS-B.
• NCBI Gene Id: 20823
• Protein Name: Lupus La protein homolog
• Description of Target: Binds to the 3' poly(U) terminus of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation.
• Uniprot ID: P32067
• Protein Accession #: NP_001103615.1
• Nucleotide Accession #: NM_001110145.1
• Protein Size (# AA): 415