SSB ELISA Kit (Human) (OKEH05884)

Catalog No: OKEH05884

Size:96T

Human SSB ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology SSB ELISA Kit (Human) (OKEH05884) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SSB has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for SSB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SSB captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 86%
• ELISA Kit Reproducibility: Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
  Mean Intra-Assay Precision - <6.2%, n = 20
  Mean Inter-Assay Precision - <8.6%, n = 20
• ELISA Kit Component:
  • SSB Microplate: 96 Wells (12 x 8 Well strips)
  • SSB Lyophilized Standard: 2
  • 100X Biotinylated SSB Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.089 ng/mL
• Specificity: Natural and recombinant Human Lupus La protein
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SSB
• Gene Full Name: small RNA binding exonuclease protection factor La
• Alias Symbols: autoantigen La;La;la autoantigen;La ribonucleoprotein;La ribonucleoprotein domain family, member 3;La/SSB;LARP3;lupus La antigen;lupus La protein;sjoegren syndrome type B antigen;Sjogren syndrome antigen B;SS-B;SS-B/La protein.
• NCBI Gene Id: 6741
• Protein Name: P05455
• Description of Target: Lupus La protein
• Uniprot ID: https://www.uniprot.org/uniprot/P05455
• Protein Accession #: NP_001281074.1
• Nucleotide Accession #: NM_001294145.1
• Protein Size (# AA): 408