CCL2 Antibody (OASA07962)

Catalog No: OASA07962 (formerly GWB-53C5C7)

Size:0.1mg

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified IgG - lyophilised
• Clonality: Polyclonal
• Isotype: Polyclonal IgG
• Host: Rabbit
• Application: WB
  Preservative Stabilisers: None present
  Antiserum Preparation: Antisera to human MCP-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
  Approx Protein Conc: IgG concentration 1.0 mg/ml
  Buffer Solutions: Phosphate buffered saline pH7.4
• Reconstitution and Storage: Reconstitution: Reconstitute with 0.1 ml distilled water.
  For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
  Storage: Prior to reconstitution store at +4^oC. Following reconstitution store at -20^oC.
  This product should be stored undiluted.
  Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
  
• Immunogen: The immunogen for anti-CCL2 antibody: recombinant human MCP-1
• Predicted Homology Based on Immunogen Sequence: Human
• Specificity: MCP-1
• Application Info: ELISA:    0.5ug/ml
  Western Blotting:    0.1ug/ml - 0.2ug/ml
  Functional Assays:    2.0ug/ml - 3.0ug/ml
  following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.

  	Yes	No	Not Determined	Suggested Dilution
  Flow Cytometry
  Immunohistology - Frozen
  Immunohistology - Paraffin
  Immunohistology - Resin
  ELISA				0.5ug/ml
  Immunoprecipitation
  Western Blotting				0.1ug/ml - 0.2ug/ml
  Functional Assays				2.0ug/ml - 3.0ug/ml

  Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using apropriate negative/positive controls.
• Reference: 1. Banisor, I. et al. (2005) Involvement of beta-chemokines in the development of inflammatory demyelination. J. Neuroinflammation. 2: 7-20.
  

Target Info

• Gene Symbol: CCL2
• Alias Symbols: HC11, MCAF, MCP1, MCP-1, SCYA2, GDCF-2, SMC-CF, HSMCR30
• NCBI Gene Id: 6347
• Protein Name: C-C motif chemokine 2
• Description of Target: RABBIT ANTI HUMAN MCP-1
  
• Uniprot ID: P13500
• Protein Accession #: NP_002973.1
• Protein Size (# AA): 99