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CCL2 Antibody (OASA07962)

0.1 mg
$609.00
In Stock
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Gene Symbol:
CCL2
NCBI Gene Id:
6347
Protein Name:
C-C motif chemokine 2
Swissprot Id:
P13500
Protein Accession #:
NP_002973.1
Alias Symbols:
HC11, MCAF, MCP1, MCP-1, SCYA2, GDCF-2, SMC-CF, HSMCR30, MGC9434, CCL2
Replacement Item:
This antibody may replace item sc-130328 from Santa Cruz Biotechnology.
Description of Target:
RABBIT ANTI HUMAN MCP-1
Protein Size (# AA):
99
Host:
Rabbit
Clonality:
Polyclonal
Application:
ELISA, WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CCL2.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CCL2.
Immunogen:
The immunogen for anti-CCL2 antibody: recombinant human MCP-1
Predicted Species Reactivity:
Human
Predicted Homology Based on Immunogen Sequence:
Human
Product Format:
Purified IgG - lyophilised
Reconstitution and Storage:
Reconstitution: Reconstitute with 0.1 ml distilled water.
For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Storage: Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets/Manuals:
Printable datasheet for anti-CCL2 antibody - OASA07962
Specificity:
MCP-1
Isotype:
Polyclonal IgG
Application Info:
ELISA:    0.5ug/ml
Western Blotting:    0.1ug/ml - 0.2ug/ml
Functional Assays:    2.0ug/ml - 3.0ug/ml
following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
Flow Cytometry      
Immunohistology - Frozen      
Immunohistology - Paraffin      
Immunohistology - Resin      
ELISA    0.5ug/ml
Immunoprecipitation      
Western Blotting    0.1ug/ml - 0.2ug/ml
Functional Assays    2.0ug/ml - 3.0ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using apropriate negative/positive controls.
:::
Preservative Stabilisers: None present
Antiserum Preparation: Antisera to human MCP-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
:::
Approx Protein Conc: IgG concentration 1.0 mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
Target Reference:
1. Banisor, I. et al. (2005) Involvement of beta-chemokines in the development of inflammatory demyelination. J. Neuroinflammation. 2: 7-20.

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