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Datasheets/Manuals | Printable datasheet for anti-APOA1 antibody - OASA09127 |
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Predicted Species Reactivity | Human |
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Product Format | Purified IgG - lyophilised |
Clonality | Polyclonal |
Isotype | Polyclonal IgG |
Host | Sheep |
:: | Preparation: Purified IgG prepared by affinity chromatography on immobilized human apolipoprotein A1 Preservative Stabilisers: Contains mannitol, dextran & salts |
Reconstitution and Storage | Reconstitution: Use sterile DI water Storage: Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. |
Immunogen | The immunogen for anti-APOA1 antibody: purified human apolipoprotein A1 |
Predicted Homology Based on Immunogen Sequence | Human |
Specificity | APOLIPOPROTEIN A1 |
Application Info | ELISA : This antibody can be used in a sandwich ELISA with OASA00955 as the capture antibody. |
Protocol Information | Citation: 1: Sonoyama K, Nishikawa H, Kiriyama S, Niki R. Apolipoprotein mRNA in liver and intestine of rats is affected by dietary beet fiber or cholestyramine. J Nutr.1995 Jan;125(1):13-9. PubMed PMID: 7815170. Species: Rat Experiment Name: Immunoblotting for plasma apo A-I quantification Experiment Background: In this study, Kei et al. have collected data from the liver and examined the effects of diets containing beet fiber, cholestyramine and no fiber (fiber-free) on plasma and liver lipids and tissue apolipoprotein mRNA. Experimental Steps: Whole plasma (0.5 uL) was subjected to 12% SDS-PAGE under reducing conditions (Laemmli 1970) and then electrophoretically transferred to nitrocellulose membrane (Hybond C extra, Amersham Inter national, Amersham, U.K.) in a semidry electroblotting apparatus (Nippon Eido, Tokyo, Japan) at constant current of 170 mA for 1 h. The blotting buffer contained 125 mmol/L Tris, 960 mmol/L glycine in 200 mL/L methanol, pH 8.3. The membrane was then incubated in a blocking solution of 50 g/L bovine serum albumin (fraction V, Miles Inc., Kankakee, IL) in Tris-buffered saline (20 mmol/L Tris-HC1, 150 mmol/L NaCl, pH 7.4), followed by the incubation for 1 h with a 1:100 dilution of the sheep anti-human apo A-I serum (Oxford, U.K.) in the blocking solution containing 500 mg/L Tween 20. The membrane was then washed three times with Tris-buffered saline containing 500 mg/L Tween 20. Next, the membrane was incubated for 1 h with a 1:2000 dilution of a second antibody of rabbit antisheep IgG conjugated with horseradish peroxidase (Jackson Immunoresearch Laboratories, West Grove, PA) in the same solution used in the incubation with the first antibody, followed by washing in the sameway with the first antibody. Identification of the antigen-antibody complex was performed using Renaissance Western Blot Chemiluminescence Reagent (DuPont NEN Research Products, Boston, MA) as recommended by the manufacturer. The relative quantity of apo A-I was estimated by scanning densitometry (Dual-Wavelength Flying-Spot Scanner CS-9000, Shimadzu, Kyoto, Japan). Number Of Protocols: 1 |
Gene Symbol | APOA1 |
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Alias Symbols | HPALP2, apo(a) |
NCBI Gene Id | 335 |
Protein Name | Apolipoprotein A-I |
Description of Target | SHEEP ANTI HUMAN APOLIPOPROTEIN A1 |
Uniprot ID | P02647 |
Protein Accession # | NP_000030.1 |
Protein Size (# AA) | 267 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "APOA1 Antibody (OASA09127)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "APOA1 Antibody (OASA09127)"?
This item is available "Domestic: within 1 week delivery | International: 1 week".
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What buffer format is "APOA1 Antibody (OASA09127)" provided in?
This item is provided in "Purified IgG - lyophilised ".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "APOA1 Antibody (OASA09127)"?
This target may also be called "HPALP2, apo(a)" in publications.
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What is the shipping cost for "APOA1 Antibody (OASA09127)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "APOA1 Antibody (OASA09127)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "APOA1 Antibody (OASA09127)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "APOA1 Antibody (OASA09127)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "APOA1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "APOA1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "APOA1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "APOA1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "APOA1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "APOA1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.