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APOA1 Antibody (OASA09127)

0.5 mg
$575.00
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Gene Symbol:
APOA1
NCBI Gene Id:
335
Protein Name:
Apolipoprotein A-I
Swissprot Id:
P02647
Protein Accession #:
NP_000030.1
Alias Symbols:
MGC117399, APOA1
Description of Target:
SHEEP ANTI HUMAN APOLIPOPROTEIN A1
Protein Size (# AA):
267
Host:
Sheep
Clonality:
Polyclonal
Application:
ELISA
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express APOA1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express APOA1.
Immunogen:
The immunogen for anti-APOA1 antibody: purified human apolipoprotein A1
Predicted Species Reactivity:
Human
Predicted Homology Based on Immunogen Sequence:
Human
Product Format:
Purified IgG - lyophilised
Reconstitution and Storage:
Reconstitution: Use sterile DI water
Storage: Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Datasheets/Manuals:
Printable datasheet for anti-APOA1 antibody - OASA09127
Specificity:
APOLIPOPROTEIN A1
Isotype:
Polyclonal IgG
Application Info:
ELISA : This antibody can be used in a sandwich ELISA with OASA00955 as the capture antibody.
:::
Preparation: Purified IgG prepared by affinity chromatography on immobilized human apolipoprotein A1
Preservative Stabilisers: Contains mannitol, dextran & salts
Protocol Information:
Citation: 1: Sonoyama K, Nishikawa H, Kiriyama S, Niki R. Apolipoprotein mRNA in liver and intestine of rats is affected by dietary beet fiber or cholestyramine. J Nutr.1995 Jan;125(1):13-9. PubMed PMID: 7815170.
Species: Rat
Experiment Name: Immunoblotting for plasma apo A-I quantification
Experiment Background: In this study, Kei et al. have collected data from the liver and examined the effects of diets containing beet fiber, cholestyramine and no fiber (fiber-free) on plasma and liver lipids and tissue apolipoprotein mRNA.
Experimental Steps: Whole plasma (0.5 µL) was subjected to 12% SDS-PAGE under reducing conditions (Laemmli 1970) and then electrophoretically transferred to nitrocellulose membrane (Hybond C extra, Amersham Inter national, Amersham, U.K.) in a semidry electroblotting apparatus (Nippon Eido, Tokyo, Japan) at constant current of 170 mA for 1 h. The blotting buffer contained 125 mmol/L Tris, 960 mmol/L glycine in 200 mL/L methanol, pH 8.3. The membrane was then incubated in a blocking solution of 50 g/L bovine serum albumin (fraction V, Miles Inc., Kankakee, IL) in Tris-buffered saline (20 mmol/L Tris-HC1, 150 mmol/L NaCl, pH 7.4), followed by the incubation for 1 h with a 1:100 dilution of the sheep anti-human apo A-I serum (Oxford, U.K.) in the blocking solution containing 500 mg/L Tween 20. The membrane was then washed three times with Tris-buffered saline containing 500 mg/L Tween 20. Next, the membrane was incubated for 1 h with a 1:2000 dilution of a second antibody of rabbit antisheep IgG conjugated with horseradish peroxidase (Jackson Immunoresearch Laboratories, West Grove, PA) in the same solution used in the incubation with the first antibody, followed by washing in the sameway with the first antibody. Identification of the antigen-antibody complex was performed using Renaissance Western Blot Chemiluminescence Reagent (DuPont NEN Research Products, Boston, MA) as recommended by the manufacturer. The relative quantity of apo A-I was estimated by scanning densitometry (Dual-Wavelength Flying-Spot Scanner CS-9000, Shimadzu, Kyoto, Japan).
Number Of Protocols: 1

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