APOA1 Antibody - N-terminal region (OAAB04428)

Catalog No: OAAB04428

Size:400UL

Western blot analysis of anti-APOA1 Antibody (N-term) in HepG2 cell lysates (35ug/lane). APOA1(arrow) was detected using the purified Pab.

Western blot analysis of APOA1 (arrow) using rabbit polyclonal APOA1 Antibody (N-term) (Cat.#OAAB04428). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APOA1 gene.

APOA1 is strongly supported by BioGPS gene expression data to be expressed in HepG2

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with APOA1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Formalin-fixed and paraffin-embedded human Placenta tissue reacted with APOA1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: IHC-P,WB
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This APOA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-37 amino acids from the N-terminal region of human APOA1.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  APOA1 is strongly supported by BioGPS gene expression data to be expressed in HepG2

• Application Info: WB: 1:1000
  IHC-P: 1:10~50

Target Info

• Gene Symbol: APOA1
• Alias Symbols: apo-AI, apo(a), apolipoprotein A-I, Apolipoprotein A1, epididymis secretory sperm binding protein, HPALP2.
• NCBI Gene Id: 335
• Protein Name: Apolipoprotein A-I|apolipoprotein A-I isoform 1 preproprotein [Homo sapiens]
• Description of Target: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
• Uniprot ID: P02647
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_000030.1
• Molecular Weight: 30778