- Description of Target:
- p65 is a subunit of the nuclear factor kappa-B, a second messenger, which activates the transcription of a number of genes in multiple tissues. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-1760 M62399.1 8-1767
- Gene Symbol:
- RELA
- Official Gene Full Name:
- V-rel reticuloendotheliosis viral oncogene homolog A (avian)
- Alias Symbols:
- MGC131774; NFKB3; p65
- Tissue Tool:
- Find tissues and cell lines supported to express RELA.
- Protein Accession# :
- NP_068810
- Nucleotide Accession#:
- NM_021975
- Swissprot Id:
- Q04206
- Host:
- Rabbit
- Clonality:
- Polyclonal
- Protein Size (# AA):
- 551
- Molecular Weight:
- 60kDa
- Application:
- WB
- Partner Proteins:
- CRYAA, CRYAB, BAD, BAD, BAD, BAX, BAX, BAX, BBC3, BBC3, BBC3, BCL2L11, BCL2L11, BID, BID, BID, BID, BID, BIK, BIK, BMF, BMF, HRK, HRK, HRK, MOAP1, RTN1, RTN4, TP53, TPT1, ANTXR1, APAF1, AVEN, BAD, BAG1, BAK1, BAX, BBC3, BCAP31, BCL2, BCL2L1, BCL2L10, BCL2L11, BCL2L12, BCL2L14, BCLAF1, BECN1, BID, BIK, B
- Immunogen:
- The immunogen for anti-RELA antibody: synthetic peptide directed towards the middle region of human RELA
- Product Format:
- Lyophilized powder
- Purification:
- Affinity Purified
- Datasheets / Downloads:
- Printable datasheet for
anti-RELA antibody
- ARP30371_P050 - Peptide Sequence:
- Synthetic peptide located within the following region: LVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS
- Blocking Peptide:
- For anti-RELA antibody is Catalog # AAP30371 (Previous Catalog # AAPS08809)
- Key Reference:
- Wittwer,T. (2008) Biochem. Biophys. Res. Commun. 371 (2), 294-297
- Reconstitution and Storage:
- Add 50 μl of distilled water. Final anti-RELA antibody concentration is 1 mg/ml in PBS buffer. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
Product Protocols: RELA antibody tested with Human Transfected 293T Cells (ARP30371_P050)
Aviva Systems Biology is the original manufacturer of this RELA antibody (ARP30371_P050)
Click here to view the RELA antibody Western Blot Protocol
Product Datasheet Link: RELA antibody (ARP30371_P050)
WB Suggested Anti-RELA Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Transfected 293T
Western Blot image: 
Description of Target: p65 is a subunit of the nuclear factor kappa-B, a second messenger, which activates the transcription of a number of genes in multiple tissues. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-1760 M62399.1 8-1767
Questions pertaining to this data can be directed to techsupport@avivasysbio.com
Aviva Systems Biology’s RELA antibody (ARP30371_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at info@avivasysbio.com.
To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to info@avivasysbio.com. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.
All of Aviva’s products are guaranteed for the applications and experimental sample types mentioned in the datasheet below. Are you curious if this product will work for you? Please contact us at techsupport@avivasysbio.com
- Protocol:
- Tips Information:
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