PTPN6 ELISA Kit (Human) (OKCD00911)

Catalog No: OKCD00911 (Formerly GWB-KBBIK7)

Size:96WELLS

Human PTPN6 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PTPN6 Microplate: 96 Wells (12 x 8 Well Strips)
  • PTPN6 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated PTPN6 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.105 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6). No significant cross-reactivity or interference between Protein Tyrosine Phosphatase, Non Receptor Type 6 (PTPN6) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: PTPN6
• Gene Full Name: protein tyrosine phosphatase non-receptor type 6
• Alias Symbols: HCP;HCPH;hematopoietic cell phosphatase;hematopoietic cell protein-tyrosine phosphatase;HPTP1C;protein-tyrosine phosphatase 1C;protein-tyrosine phosphatase SHP-1;PTP-1C;SHP1;SHP-1;SHP-1L;SH-PTP1;tyrosine-protein phosphatase non-receptor type 6.
• NCBI Gene Id: 5777
• Protein Name: Tyrosine-protein phosphatase non-receptor type 6
• Description of Target: Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.
• Uniprot ID: P29350
• Protein Accession #: NP_002822
• Nucleotide Accession #: NM_002831.5
• Protein Size (# AA): 595