SAVE NOW with 10% off all Recombinant Antibodies Shop Now

Catalog No: OKCD02748
Size:96WELLS
Price: $700.00
SKU
OKCD02748
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for NAGA ELISA Kit (Mouse) (OKCD02748)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)92-103%93-101%78-93%83-97%
EDTA Plasma (n=5)81-95%78-92%78-98%86-93%
Heparin Plasma (n=5)79-89%82-92%90-104%79-92%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Acetylgalactosaminidase Alpha (NAGa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Acetylgalactosaminidase Alpha (NAGa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Acetylgalactosaminidase Alpha (NAGa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of N-Acetylgalactosaminidase Alpha (NAGa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)78-9583
EDTA Plasma (n=5)79-9583
Heparin Plasma(n=5)95-10299
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level N-Acetylgalactosaminidase Alpha (NAGa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level N-Acetylgalactosaminidase Alpha (NAGa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-NAGA Microplate96 Wells (12 x 8 Well strips)
NAGA Lyophilized Standard2
100X Biotinylated NAGA Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity0.125 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of N-Acetylgalactosaminidase Alpha (NAGa). No significant cross-reactivity or interference between N-Acetylgalactosaminidase Alpha (NAGa) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolNaga
Gene Full NameN-acetyl galactosaminidase, alpha
Alias Symbolsalpha-galactosidase B;alpha-N-acetylgalactosaminidase.
NCBI Gene Id17939
Protein NameAlpha-N-acetylgalactosaminidase
Description of TargetRemoves terminal alpha-N-acetylgalactosamine residues from glycolipids and glycopeptides. Required for the breakdown of glycolipids (By similarity).
Uniprot IDQ9QWR8
Protein Accession #NP_032695.3
Nucleotide Accession #NM_008669.4
Protein Size (# AA)415
Write Your Own Review
You're reviewing:NAGA ELISA Kit (Mouse) (OKCD02748)
Your Rating
We found other products you might like!