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Catalog No: OKEH03632
Size:96T
Price: $625.00
SKU
OKEH03632
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology NAGA ELISA Kit (Rat) (OKEH03632) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Naga has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Naga is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Naga captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Component
ComponentAmount
Naga Microplate96 Wells (12 x 8 Well strips)
Naga Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Naga Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.166 ng/mL
SpecificityNatural and recombinant Rat Alpha-N-acetylgalactosaminidase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolNaga
Gene Full Namealpha-N-acetylgalactosaminidase
Alias Symbolsalpha-galactosidase B;alpha-N-acetylgalactosaminidase;N-acetyl galactosaminidase, alpha.
NCBI Gene Id315165
Protein NameQ66H12
Description of TargetAlpha-N-acetylgalactosaminidase
Uniprot IDhttps://www.uniprot.org/uniprot/Q66H12
Protein Accession #NP_001012120.1
Nucleotide Accession #NM_001012120.1
Protein Size (# AA)415
Molecular Weight47 kDa
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