HSP25 Antibody (OAAD00161)

Catalog No: OAAD00161

Size:100 ug

Product Info

• Predicted Species Reactivity: Human
• Clonality: Monoclonal
• Clone: SJ-25
• Isotype: IgG1
• Host: Mouse
• Application: WB, IHC, IP, ICC
• Reconstitution and Storage: At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for longer time.
• Immunogen: Partially purified inhibitor of actin polymerization (IAP) protein from turkey gizzard smooth muscle.
• Purification: Purified by the goat anti-mouse IgG Affinity chromatography.
• Specificity: Human.
  No cross reactivity with other proteins.
• Formulation: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.01mg NaN3 as preservative.
• Application Info: Western blot At 0.5-2ug/ml with the appropriate system to detect HSP25 in cells and tissues.
  Immunohistochemistry(P) At 1-2ug/ml to detect HSP25 in formalin fixed and paraffin embedded tissues. Boiling the sections may improve the staining.
  Immunohistochemistry(F) At 1-2ug/ml to detect HSP25 in formalin or acetone fixed tissues.
  Immunocytochemistry Suitable Other applications have not been tested.
  Optimal dilutions should be determined by end user.
• Storage Buffer: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
• Reference: 1. Evgrafov, O. V.; Mersiyanova, I.; Irobi, J.; Van Den Bosch, L.; Dierick, I.; Leung, C. L.; Schagina, O.; Verpoorten, N.; Van Impe, K.; Fedotov, V.; Dadali, E.; Auer-Grumbach, M.; and 14 others : Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. Nature Genet. 36: 602-606, 2004.
  2 Wyttenbach, A.; Sauvageot, O.; Carmichael, J.; Diaz-Latoud, C.; Arrigo, A.-P.; Rubinsztein, D.C. :Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin. Hum. Molec. Genet. 11: 1137-1151, 2002.

Target Info

• Gene Symbol: HSPB1
• Alias Symbols: CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102
• NCBI Gene Id: 3315
• Protein Name: Heat shock protein beta-1
• Description of Target: The heat-shock proteins (HSPs) belong to a larger group of polypeptides, the stress proteins, that are induced in various combinations in response to environmental challenges and developmental transitions. Heat-shock 27-kD protein1also knows as HSPB. Synthesis of the small (27-kD) HSP has been shown to be correlated with the acquisition of thermotolerance. HSP27 gene is mapped to 7q11.23. Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin.
• Uniprot ID: P04792