BRCA2 Antibody (OAAF02295)

Catalog No: OAAF02295 (Formerly GWB-ASD539)

Size:100 ug

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using BRCA2 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human BRCA2.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: BRCA2 antibody detects endogenous levels of total BRCA2 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100; ELISA: 1:20000
• Reference: Wooster R., Nature 378:789-792(1995).
  Tavtigian S.V., Nat. Genet. 12:333-337(1996).
  Dunham A., Nature 428:522-528(2004).

Target Info

• Gene Symbol: BRCA2
• Gene Full Name: BRCA2 DNA repair associated
• Alias Symbols: BRCA1/BRCA2-containing complex, subunit 2;BRCC2;breast and ovarian cancer susceptibility gene, early onset;breast and ovarian cancer susceptibility protein 2;breast cancer 2 tumor suppressor;breast cancer 2, early onset;breast cancer type 2 susceptibility protein;BROVCA2;FACD;FAD;FAD1;FANCD;FANCD1;Fanconi anemia group D1 protein;GLM3;mutant BRCA2;PNCA2;truncated BRCA2 DNA repair associated;truncated BRCA2 DNA repair-associated protein;truncated breast cancer 2;truncated DNA repair-associated BRCA2;truncated DNA repair-associated protein;XRCC11.
• NCBI Gene Id: 675
• Protein Name: Breast cancer type 2 susceptibility protein
• Description of Target: Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and SEM1, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability. Silencing of BRCA2 promotes R-loop accumulation at actively transcribed genes in replicating and non-replicating cells, suggesting that BRCA2 mediates the control of R-loop associated genomic instability, independently of its known role in homologous recombination (PubMed:24896180).
• Uniprot ID: P51587
• Protein Accession #: NP_000050.2
• Molecular Weight: 384 kDa