BRCA2 Antibody (Phospho-Ser3291) (OASG00824)

Catalog No: OASG00824

Size:100 uL

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, ELISA
• Additional Information: Cellular Localization: Nucleus, Cytoplasm, Cytoskeleton, Microtubule Organizing Center, Centrosome
  Tissue Specificity: Highest levels of expression in breast and thymus, with slightly lower levels in lung, ovary and spleen.
• Reconstitution and Storage: Store at -20°C. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived from human BRCA2 around the phosphorylation site of S3291. Location: 3230-3310aa.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Concentration: 1 mg/ml
• Target Post-Translational Modification: Phospho-Ser3291
• Specificity: Phospho-BRCA2 (S3291) Polyclonal Antibody detects endogenous levels of BRCA2 protein only when phosphorylated at S3291.
• Application Info: WB: 1:500~2000
  ELISA: 1:10000
• Description: Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks.together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and SEM1, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability. Silencing of BRCA2 promotes R-loop accumulation at actively transcribed genes in replicating and non-replicating cells, suggesting that BRCA2 mediates the control of R-loop associated genomic instability, independently of its known role in homologous recombination.

Target Info

• Gene Symbol: BRCA2
• Gene Full Name: breast cancer 2, early onset
• Alias Symbols: FAD, FACD, FAD1, GLM3, BRCC2, FANCD, PNCA2, FANCD1, XRCC11, BROVCA2
• NCBI Gene Id: 675; 12190;
• Uniprot ID: P51587, P97929, O35923
• Molecular Weight: 385 kDa