YWHAB Antibody - C-terminal region (OAAB17541)

Catalog No: OAAB17541

Size:400ul

Western blot analysis of YWHAB (arrow) using rabbit polyclonal YWHAB Antibody (C-term) (Cat. # OAAB17541). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the YWHAB gene.

There is BioGPS gene expression data showing that YWHAB is expressed in HEK293, Jurkat

The anti-YWHAB Pab (Cat. # OAAB17541) is used in Western blot to detect YWHAB in Jurkat cell lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Confocal immunofluorescent analysis of YWHAB Antibody (C-term) (Cat# OAAB17541) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: IHC, WB, IF
• Additional Information: Precautions: YWHAB Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2
  Cellular Location: Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This YWHAB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-246 amino acids from the C-terminal region of human YWHAB.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  There is BioGPS gene expression data showing that YWHAB is expressed in HEK293, Jurkat

• Application Info: IHC-P: 1:50~100
  WB: 1:1000
  IF: 1:10~50

Target Info

• Gene Symbol: YWHAB
• Alias Symbols: HS1, GW128, YWHAA, KCIP-1, HEL-S-1
• NCBI Gene Id: 7529
• Protein Name: 14-3-3 protein beta/alpha
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: P31946
• Protein Accession #: NP_003395.1
• Molecular Weight: 28082 Da