- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for Anti-14-3-3 beta Polyclonal Antibody (OAAI00174) |
---|
Predicted Species Reactivity | Human|Mouse|Rat |
---|---|
Product Format | Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot |
Additional Information | Background: The 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. 14.3.3 beta is highly expressed in brain tissue. It is critical to cell transformation and mitotic signaling. |
:: | Positive Control: 3T3, Brain tissue |
:: | Cellular Location: Cytoplasmic |
Reconstitution and Storage | The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles. |
Immunogen | A synthetic peptide corresponding to N-term of human 14.3.3 beta protein. |
Purification | Affinity Purified |
Concentration | 100ug/200ul |
Formulation | This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer |
Application Info | WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested |
Reference | Zhi-jun Luo, et al. Identification of the 14.3.3 Domains Important for Self-association and Raf Binding. J. Bio. Chem 270 (40), 23681–23687, 1995 |
Gene Symbol | YWHAB |
---|---|
Gene Full Name | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta |
Alias Symbols | 14-3-3 alpha;14-3-3 protein beta/alpha;epididymis secretory protein Li 1;GW128;HEL-S-1;HS1;KCIP-1;protein 1054;protein kinase C inhibitor protein-1;tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide;tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide;YWHAA. |
NCBI Gene Id | 7529 |
Description of Target | The 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. 14.3.3 beta is highly expressed in brain tissue. It is critical to cell transformation and mitotic signaling. |
Uniprot ID | P31946 |
Molecular Weight | 28 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
-
What is the species homology for "Anti-14-3-3 beta Polyclonal Antibody (OAAI00174)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
-
How long will it take to receive "Anti-14-3-3 beta Polyclonal Antibody (OAAI00174)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
-
What buffer format is "Anti-14-3-3 beta Polyclonal Antibody (OAAI00174)" provided in?
This item is provided in "Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Anti-14-3-3 beta Polyclonal Antibody (OAAI00174)"?
This target may also be called "14-3-3 alpha;14-3-3 protein beta/alpha;epididymis secretory protein Li 1;GW128;HEL-S-1;HS1;KCIP-1;protein 1054;protein kinase C inhibitor protein-1;tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide;tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide;YWHAA." in publications.
-
What is the shipping cost for "Anti-14-3-3 beta Polyclonal Antibody (OAAI00174)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
-
What is the guarantee for "Anti-14-3-3 beta Polyclonal Antibody (OAAI00174)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
-
Can I get bulk pricing for "Anti-14-3-3 beta Polyclonal Antibody (OAAI00174)"?
You can get bulk pricing for this item by going here.
-
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "28".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Anti-14-3-3 beta Polyclonal Antibody (OAAI00174)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
-
What are positive controls for "YWHAB"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What are negative controls for "YWHAB"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What other proteins interact with "YWHAB"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
-
What biological processes are associated with "YWHAB"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
-
What cellular components are associated with "YWHAB"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
-
What protein functions are associated with "YWHAB"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.