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Datasheets/Manuals | Printable datasheet for TOP2A Antibody (OAAF02136) |
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Predicted Species Reactivity | Human|Monkey |
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Product Format | Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human TOP2A. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | TOP2A antibody detects endogenous levels of total TOP2A protein. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | WB 1:500~1000 IHC 1:50~100 IF 1:100~500 ELISA 1:20000 |
Reference | Tsai-Pflugfelder M., Proc. Natl. Acad. Sci. U.S.A. 85:7177-7181(1988). Wasserman R.A., Cancer Res. 53:3591-3596(1993). Lang A.J., Gene 221:255-266(1998). |
Gene Symbol | TOP2A |
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Gene Full Name | DNA topoisomerase II alpha |
Alias Symbols | DNA gyrase;DNA topoisomerase (ATP-hydrolyzing);DNA topoisomerase 2-alpha;DNA topoisomerase II, 170 kD;DNA topoisomerase II, alpha isozyme;TOP2;TOP2alpha;TOPIIA;topoisomerase (DNA) II alpha 170kDa;TP2A. |
NCBI Gene Id | 7153 |
Protein Name | DNA topoisomerase 2-alpha |
Description of Target | Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity). |
Uniprot ID | P11388 |
Protein Accession # | NP_001058.2 |
Molecular Weight | 174 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "TOP2A Antibody (OAAF02136)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Monkey".
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How long will it take to receive "TOP2A Antibody (OAAF02136)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "TOP2A Antibody (OAAF02136)" provided in?
This item is provided in "Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "TOP2A Antibody (OAAF02136)"?
This target may also be called "DNA gyrase;DNA topoisomerase (ATP-hydrolyzing);DNA topoisomerase 2-alpha;DNA topoisomerase II, 170 kD;DNA topoisomerase II, alpha isozyme;TOP2;TOP2alpha;TOPIIA;topoisomerase (DNA) II alpha 170kDa;TP2A." in publications.
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What is the shipping cost for "TOP2A Antibody (OAAF02136)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "TOP2A Antibody (OAAF02136)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "TOP2A Antibody (OAAF02136)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "174 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "TOP2A Antibody (OAAF02136)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "TOP2A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "TOP2A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "TOP2A"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "TOP2A"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "TOP2A"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "TOP2A"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.