TOP2A Antibody - C-terminal region (AVARP04007_T100)

Catalog No: AVARP04007_T100

WB Suggested Anti-TOP2A Antibody Titration: 1.25ug/ml
ELISA Titer: 1:62500
Positive Control: HepG2 cell lysateTOP2A is supported by BioGPS gene expression data to be expressed in HepG2

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Pig, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human TOP2A
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 87%; Pig: 100%; Rabbit: 100%; Rat: 93%
• Peptide Sequence: Synthetic peptide located within the following region: VSKAVTSKKSKGESDDFHMDFDSAVAPRAKSVRAKKPIKYLEESDEDDLF
• Concentration: 1.0 mg/ml
• Blocking Peptide: For anti-TOP2A (AVARP04007_T100) antibody is Catalog # AAP30464 (Previous Catalog # AAPP01048)
• Sample Type Confirmation:

  TOP2A is supported by BioGPS gene expression data to be expressed in HepG2

• Reference: Yoshida,K., et al., (2006) Mol. Cell. Biol. 26 (9), 3414-3431

Target Info

• Gene Symbol: TOP2A
• Gene Full Name: Topoisomerase (DNA) II alpha 170kDa
• Alias Symbols: TOP2, TP2A, TOPIIA, TOP2alpha
• NCBI Gene Id: 7153
• Protein Name: DNA topoisomerase 2-alpha
• Description of Target: TOP2A is a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.
• Uniprot ID: P11388
• Protein Accession #: NP_001058
• Nucleotide Accession #: NM_001067
• Protein Size (# AA): 1531
• Molecular Weight: 174kDa
• Protein Interactions: UBC; SUMO2; SUMO3; EIF6; RPA3; RPA2; RPA1; SUMO1; LMNA; CTNNB1; ITGA4; FN1; CSNK2A1; VCAM1; SRPK1; RB1; ESR1; TOP2B; CSNK2A2; UPF3B; PRKCG; PRKCB; PRKCA; TERF2; BLM; NEDD8; COPS5; CUL5; FBXW7; ISG15; CUL2; VHL; TCEB1; GSK3B; SUMO4; SMARCAD1; CTCF; RING1;