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Datasheets/Manuals | Printable datasheet for anti-TOP2A (AVARP04007_T100) antibody |
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Tested Species Reactivity | Human |
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Predicted Species Reactivity | Human, Mouse, Rat, Dog, Guinea Pig, Horse, Pig, Rabbit |
Product Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Clonality | Polyclonal |
Host | Rabbit |
Application | WB |
Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | The immunogen is a synthetic peptide directed towards the C terminal region of human TOP2A |
Purification | Protein A purified |
Predicted Homology Based on Immunogen Sequence | Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 87%; Pig: 100%; Rabbit: 100%; Rat: 93% |
Peptide Sequence | Synthetic peptide located within the following region: VSKAVTSKKSKGESDDFHMDFDSAVAPRAKSVRAKKPIKYLEESDEDDLF |
Concentration | 1.0 mg/ml |
Blocking Peptide | For anti-TOP2A (AVARP04007_T100) antibody is Catalog # AAP30464 (Previous Catalog # AAPP01048) |
Sample Type Confirmation | TOP2A is supported by BioGPS gene expression data to be expressed in HepG2 |
Reference | Yoshida,K., et al., (2006) Mol. Cell. Biol. 26 (9), 3414-3431 |
Gene Symbol | TOP2A |
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Gene Full Name | Topoisomerase (DNA) II alpha 170kDa |
Alias Symbols | TOP2, TP2A, TOPIIA, TOP2alpha |
NCBI Gene Id | 7153 |
Protein Name | DNA topoisomerase 2-alpha |
Description of Target | TOP2A is a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. |
Uniprot ID | P11388 |
Protein Accession # | NP_001058 |
Nucleotide Accession # | NM_001067 |
Protein Size (# AA) | 1531 |
Molecular Weight | 174kDa |
Protein Interactions | UBC; SUMO2; SUMO3; EIF6; RPA3; RPA2; RPA1; SUMO1; LMNA; CTNNB1; ITGA4; FN1; CSNK2A1; VCAM1; SRPK1; RB1; ESR1; TOP2B; CSNK2A2; UPF3B; PRKCG; PRKCB; PRKCA; TERF2; BLM; NEDD8; COPS5; CUL5; FBXW7; ISG15; CUL2; VHL; TCEB1; GSK3B; SUMO4; SMARCAD1; CTCF; RING1; |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "TOP2A Antibody - C-terminal region (AVARP04007_T100)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human, Mouse, Rat, Dog, Guinea Pig, Horse, Pig, Rabbit".
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How long will it take to receive "TOP2A Antibody - C-terminal region (AVARP04007_T100)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
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What buffer format is "TOP2A Antibody - C-terminal region (AVARP04007_T100)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "TOP2A Antibody - C-terminal region (AVARP04007_T100)"?
This target may also be called "TOP2, TP2A, TOPIIA, TOP2alpha" in publications.
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What is the shipping cost for "TOP2A Antibody - C-terminal region (AVARP04007_T100)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "TOP2A Antibody - C-terminal region (AVARP04007_T100)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "TOP2A Antibody - C-terminal region (AVARP04007_T100)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "174kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "TOP2A Antibody - C-terminal region (AVARP04007_T100)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "TOP2A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "TOP2A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "TOP2A"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "TOP2A"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "TOP2A"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "TOP2A"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.