SOX4 Antibody - N-terminal region (OAAB15658)

Catalog No: OAAB15658

Size:400ul

Western blot analysis of SOX4 antibody (N-term) (Cat. #OAAB15658) in A2058 cell line lysates (35ug/lane). SOX4 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain reacted with SOX4 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Fluorescent confocal image of Hela cell stained with SOX4 Antibody (N-term)(Cat# OAAB15658).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX4 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SOX4 immunoreactivity is localized to Mitochondria significantly and Nucleus weakly.

SOX4 Antibody (N-term) (Cat. #OAAB15658) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: IF, WB, IHC-P, FC
  Function: Transcriptional activator that binds with high affinity to the T-cell enhancer motif 5'-AACAAAG-3' motif.
  Cellular Location: Nucleus {ECO:0000255|PROSITE- ProRule:PRU00267, ECO:0000269|PubMed:16631117}
  Tissue Location Testis, brain, and heart.
  
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This SOX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-114 amino acids from the N-terminal region of human SOX4.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Intended Use: SOX4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
  IF: 1:10~50

Target Info

• Gene Symbol: SOX4
• Alias Symbols: CSS10, EVI16
• NCBI Gene Id: 6659
• Protein Name: Transcription factor SOX-4
• Description of Target: SOX4 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The protein may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin). The protein may function in the apoptosis pathway leading to cell death as well as to tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development. The solution structure has been resolved for the HMG-box of a similar mouse protein.
• Uniprot ID: Q06945
• Protein Accession #: NP_003098.1
• Molecular Weight: 47263 Da