SLC6A3 ELISA Kit (Human) (OKCD02489)

Catalog No: OKCD02489

Size:96WELLS

Human SLC6A3 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dopamine Transporter (DAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dopamine Transporter (DAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dopamine Transporter (DAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Dopamine Transporter (DAT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dopamine Transporter (DAT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dopamine Transporter (DAT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SLC6A3 Microplate: 96 Wells (12 x 8 Well strips)
  • SLC6A3 Lyophilized Standard: 2
  • 100X Biotinylated SLC6A3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Dopamine Transporter (DAT). No significant cross-reactivity or interference between Dopamine Transporter (DAT) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: SLC6A3
• Gene Full Name: solute carrier family 6 member 3
• Alias Symbols: DA transporter;DAT;DAT1;dopamine transporter 1;PKDYS;PKDYS1;sodium-dependent dopamine transporter;solute carrier family 6 (neurotransmitter transporter), member 3;solute carrier family 6 (neurotransmitter transporter, dopamine), member 3;Solute carrier family 6 member 3.
• NCBI Gene Id: 6531
• Protein Name: Sodium-dependent dopamine transporter
• Description of Target: Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.
• Uniprot ID: Q01959
• Protein Accession #: NP_001035.1
• Nucleotide Accession #: NM_001044.4
• Protein Size (# AA): 620