S1PR5 ELISA Kit (Mouse) (OKCD02866)

Catalog No: OKCD02866

Size:96WELLS

Mouse S1PR5 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  	94-102%	99-105%	87-95%	97-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine 1 Phosphate Receptor 5 (S1PR5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sphingosine 1 Phosphate Receptor 5 (S1PR5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sphingosine 1 Phosphate Receptor 5 (S1PR5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Sphingosine 1 Phosphate Receptor 5 (S1PR5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  	90-97	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sphingosine 1 Phosphate Receptor 5 (S1PR5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sphingosine 1 Phosphate Receptor 5 (S1PR5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-S1PR5 Microplate: 96 Wells (12 x 8 Well strips)
  • S1PR5 Lyophilized Standard: 2
  • 100X Biotinylated S1PR5 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.059 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sphingosine 1 Phosphate Receptor 5 (S1PR5). No significant cross-reactivity or interference between Sphingosine 1 Phosphate Receptor 5 (S1PR5) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: S1PR5
• Gene Full Name: sphingosine-1-phosphate receptor 5
• Alias Symbols: S1, lp, Edg8, S1P5, lpB4
• NCBI Gene Id: 94226
• Protein Name: Sphingosine 1-phosphate receptor 5
• Description of Target: Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Is coupled to both the G(i/0)alpha and G(12) subclass of heteromeric G-proteins. S1P activation on oligodendroglial cells modulates two distinct functional pathways mediating either process retraction or cell survival. S1P activation on O4-positive pre-oligodendrocytes induces process retraction via a Rho kinase/collapsin response-mediated protein signaling pathway. The S1P-induced survival of mature oligodendrocytes is mediated through a pertussis toxin-sensitive, Akt-dependent pathway. S1P activation on oligodendroglial cells modulates two distinct functional pathways mediating either process retraction or cell survival. These effects depend on the developmental stage of the cell.
• Uniprot ID: Q91X56
• Protein Accession #: NP_444420.1
• Nucleotide Accession #: NM_053190.2
• Protein Size (# AA): 400