RND3 Antibody - middle region (OAAB04793)

Catalog No: OAAB04793

Size:400ul

Western blot analysis of ARHE Antibody (Center) in mouse brain tissue lysates (35ug/lane). ARHE (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ARHE antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC-P
  Function: Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins.
  Cellular Location: Golgi apparatus membrane; Peripheral membrane protein.
  Tissue Location Ubiquitous.
  
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This ARHE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-165 amino acids from the Central region of human ARHE.
• Purification: This antibody is purified by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Predicted Homology Based on Immunogen Sequence: Rat, Pig
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
  
• Application Info: WB: 1:2000
  WB: 1:1000
  IHC-P: 1:10~50

Target Info

• Gene Symbol: RND3
• Alias Symbols: ARHE, Rho8, RhoE, memB
• NCBI Gene Id: 390
• Protein Name: Rho-related GTP-binding protein RhoE
• Description of Target: Members of the Rho family of Ras-related GTPases, such as ARHE, regulate the organization of the actin cytoskeleton in response to extracellular growth factors. Like Ras (MIM 190020), Rho family members appear to cycle between an inactive GDP-bound form and an active GTP-bound form. Three major regulators of Rho activity have been identified: RhoGDIs, which interact with the GDP-bound Rho proteins to keep them in a resting complex (see MIM 601925); GEFs, which promote GDP/GTP exchange leading to activation of Rho proteins (see MIM 601855); and GAPs, which stimulate GTP hydrolysis and return the activated Rho protein to its inactive form (see MIM 602680) (Nobes et al., 1998 [PubMed 9531558]).
• Uniprot ID: P61587
• Protein Accession #: NP_001241667.1
• Molecular Weight: 27368 Da