MBD1 Antibody - C-terminal region (ARP32693_P050)

Catalog No: ARP32693_P050

WB Suggested Anti-MBD1 Antibody
Titration: 1.0 ug/ml
Positive Control: 293T Whole CellMBD1 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells

Rabbit Anti-MBD1 Antibody
Catalog Number: ARP32693_P050
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Cytoplasmic
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec

Rabbit Anti-MBD1 antibody
Catalog Number: ARP32693
Formalin Fixed Paraffin Embedded Tissue: Human Testis
Primary antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20x
Exposure Time: 0.5-2.0sec

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: EEDKEENKDDSASKLAPEEEAGGAGTPVITEIFSLGGTRFRDTAVWLPRS
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-MBD1 (ARP32693_P050) antibody is Catalog # AAP32693 (Previous Catalog # AAPP03707)
• Sample Type Confirmation:

  MBD1 is strongly supported by BioGPS gene expression data to be expressed in HEK293T

Target Info

• Gene Symbol: MBD1
• Gene Full Name: Methyl-CpG binding domain protein 1
• Alias Symbols: RFT, PCM1, CXXC3
• NCBI Gene Id: 4152
• Protein Name: Methyl-CpG-binding domain protein 1
• Description of Target: The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains multiple domains: MBD at the N-terminus that functions both in binding to methylated DNA and in protein interactions; several CXXC-type zinc finger domains that mediate binding to non-methylated CpG dinucleotides; transcriptional repression domain (TRD) at the C-terminus that is involved in transcription repression and in protein interactions. Numerous alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
• Uniprot ID: Q9UIS9
• Protein Accession #: NP_001191067
• Nucleotide Accession #: NM_001204138
• Protein Size (# AA): 629
• Molecular Weight: 69kDa
• Protein Interactions: SUMO1; HTT; UBC; ECT2; APP; ATXN1L; TENM1; SUMO2; HIST1H3A; PCNA; ATF7IP2; ATF7IP; CHAF1A; SETDB1; PIAS1; STAT1; PRAM1; HDAC3; PIAS2; ZNF512B; Rnf2; CBX4; CBX5; HIPK3; SMAD9; OASL; SMAD5; SMAD3; SMAD7; SMAD1; SUV39H1;