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Datasheets/Manuals | Printable datasheet for anti-SIRT6 (ARP32408_P050) antibody |
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Tested Species Reactivity | Human |
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Predicted Species Reactivity | Human, Rat, Cow, Dog |
Product Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Clonality | Polyclonal |
Host | Rabbit |
Application | WB, IP, CHIP |
Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | The immunogen is a synthetic peptide directed towards the middle region of human SIRT6 |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Cow: 100%; Dog: 100%; Human: 100%; Rat: 83% |
Peptide Sequence | Synthetic peptide located within the following region: TRINGSIPAGPKQEPCAQHNGSEPASPKRERPTSPAPHRPPKRVKAKAVP |
Concentration | 0.5 mg/ml |
Blocking Peptide | For anti-SIRT6 (ARP32408_P050) antibody is Catalog # AAP32408 (Previous Catalog # AAPP03403) |
Reference | de Nat. Struct. Mol. Biol. 15 (4), 336 (2008) |
Publications | Gazin, C., Wajapeyee, N., Gobeil, S., Virbasius, C.-M. & Green, M. R. An elaborate pathway required for Ras-mediated epigenetic silencing. Nature 449, 1073-7 (2007). 17960246 Wajapeyee, N., Malonia, S. K., Palakurthy, R. K. & Green, M. R. Oncogenic RAS directs silencing of tumor suppressor genes through ordered recruitment of transcriptional repressors. Genes Dev. 27, 2221-6 (2013). 24105743 |
Gene Symbol | SIRT6 |
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Gene Full Name | Sirtuin 6 |
Alias Symbols | SIR2L6 |
NCBI Gene Id | 51548 |
Protein Name | NAD-dependent protein deacetylase sirtuin-6 |
Description of Target | SIRT6 is a NAD-dependent protein deacetylase. SIRT6 has deacetylase activity towards 'Lys-9' and 'Lys-56' of histone H3. SIRT6 modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates 'Lys-9' of histone H3 at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Deacetylation of nucleosomes interferes with RELA binding to target DNA. SIRT6 may be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. SIRT6 is required for genomic stability, normal IGF1 serum levels and normal glucose homeostasis. SIRT6 modulates cellular senescence and apoptosis. SIRT6 regulates the production of TNF protein.This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. |
Uniprot ID | Q8N6T7 |
Protein Accession # | NP_057623 |
Nucleotide Accession # | NM_016539 |
Protein Size (# AA) | 355 |
Molecular Weight | 39kDa |
Protein Interactions | UBC; MDM2; AKT1; STUB1; SKP2; ABCF2; FAF1; VIM; UBE2D1; CHD3; NR0B2; tat; CCNDBP1; RELA; XRCC5; PRKDC; XRCC6; ELF5; |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "SIRT6 Antibody - middle region (ARP32408_P050)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human, Rat, Cow, Dog".
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How long will it take to receive "SIRT6 Antibody - middle region (ARP32408_P050)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
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What buffer format is "SIRT6 Antibody - middle region (ARP32408_P050)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "SIRT6 Antibody - middle region (ARP32408_P050)"?
This target may also be called "SIR2L6" in publications.
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What is the shipping cost for "SIRT6 Antibody - middle region (ARP32408_P050)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "SIRT6 Antibody - middle region (ARP32408_P050)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "SIRT6 Antibody - middle region (ARP32408_P050)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "39kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "SIRT6 Antibody - middle region (ARP32408_P050)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "SIRT6"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "SIRT6"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "SIRT6"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "SIRT6"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "SIRT6"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "SIRT6"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.