Biotinylated CCL4 Protein (OPCB00017)

Catalog No: OPCB00017

Size:2ug

Migration Assay: Cells expressing recombinant CCR5 were assayed for migration through a transwell filter at various concentrations of WT or Biotinylated MIP-1beta. Responses are expressed as the % of total input cells (Blue: wild type; Red: biotinylated).

Product Info

• Product Format: Lyophilized
• Conjugation: Biotin
• Application: CA
  Endotoxin Level: <0.01 EU per 1ug of the protein by the LAL method
• Reconstitution and Storage: Recommended at 100ug/mL in sterile water
  Shipping: Room Temp
• Purity: > 97% as determined by SDS-PAGE
• Biological Activity: EC50 = 0.32-1.3 nM determined by Migration Assay in cells expressing recombinant CCR5
• Source: E. coli
• Reference: 1. "Identification of RANTES, MIP-1 alpha, and MIP-1 beta as the major HIV-suppressive factors produced by CD8+ T cells."
  Cocchi F., DeVico A.L., Garzino-Demo A., Arya S.K., Gallo R.C., Lusso P.
  Science 270:1811-1815 (1995)
  
  2. "The assignment of chemokine-chemokine receptor pairs: TARC and MIP-1 beta are not ligands for human CC-chemokine receptor 8."
  Garlisi C.G., Xiao H., Tian F., Hedrick J.A., Billah M.M., Egan R.W., Umland S.P.
  Eur. J. Immunol. 29:3210-3215 (1999)
  3. "Natural truncation of the chemokine MIP-1beta/CCL4 affects receptor specificity but not anti-HIV-1 activity."
  Guan E., Wang J., Roderiquez G., Norcross M.A.
  J. Biol. Chem. 277:32348-32352 (2002)

Target Info

• Gene Symbol: CCL4
• Gene Full Name: chemokine (C-C motif) ligand 4-like 1
• Alias Symbols: LAG1, CCL4L, LAG-1, SCYA4L, AT744.2, SCYA4L1, SCYA4L2, MIP-1-beta
• NCBI Gene Id: 388372
• Description of Target: Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.
• Uniprot ID: P13236