CCL4/MIP-1 beta ELISA Kit (Human) (OKBB00368)

Catalog No: OKBB00368

Size:96 Wells

Human CCL4/MIP-1 beta ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human CCL4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL4 has been precoated onto 96-well plates. Standards(E.coli, A24-N92) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human CCL4 amount of sample captured in plate.
• ELISA Kit Range: 7.8 pg/ml - 500 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	32	91	324	33	87	319
  SD	1.3	3.8	15.2	2.1	5.8	24.9
  CV%	4.1	4.2	4.7	6.4	6.7	7.8

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human MIP-1 beta Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human MIP-1 beta Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Human MIP-1 beta Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
• Sensitivity: <1 pg/ml
• Specificity: Natural and recombinant human CCL4
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: A24-N92
• Description: For quantitative detection of human CCL4 in cell culture supernatants, serum and plasma(heparin, EDTA, citrate).

Target Info

• Gene Symbol: CCL4
• Gene Full Name: chemokine (C-C motif) ligand 4
• Alias Symbols: ACT 2, ACT2, AT744.1, C C motif chemokine 4, CCL4, CCL4L1, Chemokine CC Motif Ligand 4, G 26 T lymphocyte-secreted protein, HC21, LAG 1, LAG1, Lymphocyte activation gene 1, Lymphocyte activation gene 1 protein, MGC104418, MGC126025, MGC126026, MIP 1 beta, MIP1B, PAT 744, Protein H400, SCYA2, SCYA4, Secreted protein G 26, SIS gamma, Small inducible cytokine A4, T cell activation protein 2
• NCBI Gene Id: 6351
• Protein Name: C-C motif chemokine 4
• Description of Target: Chemokine (C-C motif) ligand 4, also known as CCL4, is a protein which in humans is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. the CCL4 gene was assigned to a slightly more distal location: 17q21-q23 rather than 17q11-q21.
• Uniprot ID: P13236
• Protein Accession #: NP_002975.1
• Nucleotide Accession #: NM_002984.3