Anti-Clavesin 1/2 (OAPC00092)

Catalog No: OAPC00092

Size:100 ul

Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~ 35k clavesin protein.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. 100 ul in PBS + 50% glycerol.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, ICC
• Additional Information: Packaging :
  100 ul in PBS + 50% glycerol.
• Reconstitution and Storage: Store at -20C in undiluted aliquots; stable for up at least 1 year after date of receipt. Avoid freeze/thaw cycles.
• Immunogen: Synthetic peptide taken from the C-terminus of rat clavesin 1.
• Purification: Prepared from serum by affinity purification using a column to which the peptide antigen was coupled.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Specificity: Specific for the ~ 35 kDa clavesin 1/2 protein doublet in Western blots of rat brain lysate. Isoform-specific knock down in cultured hippocampal neurons indicates that the lower and upper bands are clavesin 1 and 2, respectively.
• Application Info: The antibody has been directly tested for reactivity in rat. It is anticipated that the antibody will also work in human and mouse as there is 100% homology in these species with the amino acid sequence used as antigen.
  Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~ 35k clavesin protein.
  
• Reference: Yohei Katoh, Brigitte Ritter, Thomas Gaffry, Francois Blondeau, Stefan Höning and
  Peter S. McPherson (2009) the clavesin family: neuron-specific lipid- and clathrin-binding Sec14 proteins regulating lysosomal morphology. Journal of Biological Chemistry Oct 2;284(40):27646-54.
  Zhao S, Xu C, Qian H, Lv L, Ji C, Chen C, Zhao X, Zheng D, Gu S, Xie Y, Mao Y (2008)
  Cellular retinaldehyde-binding protein-like (CRALBPL), a novel human Sec14p-like gene
  that is upregulated in human hepatocellular carcinomas, may be used as a marker for human hepatocellular carcinomas. DNA Cell Biol. Mar; 27(3):159-63.

Target Info

• Gene Symbol: CLVS1/2
• Alias Symbols: Rlbp1l1, Clavesin-1, RGD1564200
• NCBI Gene Id: 366311
• Protein Name: Clavesin-1
• Description of Target: Clavesin (clathrin vesicle associated Sec14 protein) is a novel neuron specific protein that has recently been identified and shown to be required for normal morphology of late endosomes and/or lysosomes as lentiviral-mediated knockdown of clavesin in hippocampal neurons causes lysosomal defects (Katoh et al., 2009). Additionally, upregulation of clavesin in human hepatocellular carcinoma has recently been demonstrated thus making it a useful marker for this disease state (Zhao et al., 2008).
• Uniprot ID: A6JFQ6
• Protein Accession #: NP_001102439.1
• Molecular Weight: ~35kDa