Catalog No: OKEH04632
Size:96T
Price: $525.00
SKU
OKEH04632
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology PRAME ELISA Kit (Human) (OKEH04632) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PRAME has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for PRAME is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PRAME captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-Assay CV: < 10% Mean Inter-Assay CV: < 12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 0.056 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Melanoma antigen preferentially expressed in tumors | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | PRAME |
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Gene Full Name | PRAME nuclear receptor transcriptional regulator |
Alias Symbols | cancer/testis antigen 130;CT130;MAPE;melanoma antigen preferentially expressed in tumors;OIP4;OIP-4;opa-interacting protein 4;Opa-interacting protein OIP4;preferentially expressed antigen in melanoma;preferentially expressed antigen of melanoma. |
NCBI Gene Id | 23532 |
Protein Name | P78395 |
Description of Target | Melanoma antigen preferentially expressed in tumors |
Uniprot ID | https://www.uniprot.org/uniprot/P78395 |
Protein Accession # | NP_001278644.1 |
Nucleotide Accession # | NM_001291715.1 |
Protein Size (# AA) | 509 |
Molecular Weight | 58 KDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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