MMP14 ELISA Kit (Pig) (OKEH03937)

Catalog No: OKEH03937

Size:96T

Pig MMP14 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Porcine, Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology MMP14 ELISA Kit (Pig) (OKEH03937) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MMP14 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP14 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MMP14 captured in well.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Component:
  • MMP14 Microplate: 96 Wells (12 x 8 Well strips)
  • MMP14 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated MMP14 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.23 ng/mL
• Specificity: Natural and recombinant Pig Matrix metalloproteinase-14
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: MMP14
• Gene Full Name: matrix metallopeptidase 14
• Alias Symbols: matrix metallopeptidase 14 (membrane-inserted);matrix metalloproteinase-14;membrane-type 1 matrix metalloproteinase;membrane-type matrix metalloproteinase 1;Membrane-type-1 matrix metalloproteinase;MMP-14;MT1MMP;MT1-MMP;MT-MMP 1;MTMMP1.
• NCBI Gene Id: 397471
• Protein Name: Q9XT90
• Description of Target: Matrix metalloproteinase-14
• Uniprot ID: https://www.uniprot.org/uniprot/Q9XT90
• Protein Accession #: NP_999404.1
• Nucleotide Accession #: NM_214239.1
• Protein Size (# AA): 580
• Molecular Weight: 580 kDa