MMP14 Antibody - N-terminal region (OAAB03401)

Catalog No: OAAB03401

Size:400ul

All lanes : Anti-MMP14 Antibody at 1:2000 dilution
Lane 1: A2058 whole cell lysate
Lane 2: HT-1080 whole cell lysate
Lane 3: mouse lung lysate
Lane 4: MCF-7 whole cell lysate
Lane 5: NCI-H292 whole cell lysate
Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 66 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

OAAB03401 staining MMP14 in human lung adenocarcinoma tissue sections by Immunohistochemistry
(IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Flow cytometric analysis of MCF-7 cells using MMP14 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P, FC
• Additional Information: Function: Endopeptidase that degrades various components of the extracellular matrix such as collagen. Activates progelatinase A. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro-MMP2. Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro-MMP2.
  Cellular Location: Membrane; Single-pass type I membrane protein. Melanosome. Cytoplasm Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Forms a complex with BST2 and localizes to the cytoplasm.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This MMP14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-174 amino acids from the N-terminal region of human MMP14.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Cow, Pig, Rat
• Concentration: 0.45 mg/ml
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: IHC-P: 1:25
  FC: 1:10~50
  WB: 1:2000
  WB: 1:1000
• Other Applications Image 1 Data: Flow cytometric analysis of MCF-7 cells using MMP14 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: MMP14
• Alias Symbols: MMP-14, MMP-X1, MT-MMP, MT1MMP, MTMMP1, WNCHRS, MT1-MMP, MT-MMP 1
• NCBI Gene Id: 4323
• Protein Name: Matrix metalloproteinase-14
• Description of Target: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP14 seems to specifically activate progelatinase A, and may thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. Expression is significant in stromal cells of colon, breast, and head, neck, and lung tumors.
• Uniprot ID: P50281
• Protein Accession #: NP_004986
• Molecular Weight: 66 kDa