MAGEA4 Antibody - N-terminal region (OAAB16515)

Catalog No: OAAB16515

Size:400ul

The anti-MAGE-A4 Pab (Cat. # OAAB16515) is used in Western blot to detect MAGE-A4 in A375 cell lysate.

Immunostaining of paraformldehyde-fixed and paraffin-embedded human breast carcinoma with MAGE-A4 antibody Cat# OAAB16515 (1:50 dilution), followed by reacting with biotin-conjugated secondary antibody, ABC solution and developing with DAB. Cancerous cells show nuclear staining of MAGE-A4. Magnification: 40X. Data courtesy of Dr. Mi Hou, Karolinska?Institutet and University Hospital, Sweden.

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MAGEA4 antibody (N-term) (Cat.# OAAB16515), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Precautions: MAGEA4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression
  Tissue Location: Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This MAGEA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-38 amino acids from the N-terminal region of human MAGEA4.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: MAGEA4
• Alias Symbols: CT1.4, MAGE4, MAGE4A, MAGE4B, MAGE-41, MAGE-X2
• NCBI Gene Id: 4103
• Protein Name: Melanoma-associated antigen 4
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: P43358
• Protein Accession #: NP_001011548.1
• Molecular Weight: 34899 Da