M-CSF ELISA Kit (Mouse) (OKBB00212)

Catalog No: OKBB00212 (Formerly GWB-ZZD125)

Size:96 Tests

Mouse CSF1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's mouse M-CSF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for M-CSF has been precoated onto 96-well plates. Standards(E.coli, K33-E262) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for M-CSF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse M-CSF amount of sample captured in plate.
• ELISA Kit Range: 31.2 pg/ml - 2,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  Mean (pg/mL)	92	316	628	127	439	685
  St. Dev.	5.06	19.28	40.2	7.493	27.66	51.4
  %CV	5.5	6.10	6.40	5.9	6.30	7.50

• ELISA Kit Component:
  • Lyophilized recombinant human IL-12(p40) standard: 10 ng/tube x 2
  • Anti-human IL-12(p40) Antibody Well Plate: 96 Wells
  • Sample diluent buffer: 30 mL
  • Biotinylated anti-human IL-12(p40) antibody: 130 uL, dilution 1:100
  • Antibody diluent buffer: 12 mL
  • Avidin-Biotin-Peroxidase Complex (ABC): 130 uL, dilution 1:100
  • ABC diluent buffer: 12 mL
  • TMB color developing agent: 10 mL
  • TMB stop solution: 10 mL
• Additional Information: Range: 15.6pg/ml-1000pg/ml
  Principle: Aviva’s mouse M-CSF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse M-CSF specific-specific polyclonal antibodies were precoated onto 96-well plates. The mouse specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse M-CSF amount of sample captured in plate.
  Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
  experiment using standards and a small number of samples is recommended.
  2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
  3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
  4. Duplicate well assay is recommended for both standard and sample testing.
  5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
  6. Don’t reuse tips and tubes to avoid cross contamination.
  7. To avoid to use the reagents from different batches together.
  8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
  marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
  using.
  Background: M-CSF, also called CSF1, consists of a 14-amino acid peptide, longer than the usual 8-to-11 mer recognized by most CTLs. The M-CSF gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb1. Although it is a single-copy gene, its expression results in the synthesis of several mRNAs, ranging in size from about 1.5 to 4.5 kb2. There are 2 forms of M-CSF, with 224 and 522 amino acids, resulting from alternative splicing3. Furthermore, M-CSF has a role in development of the placenta. Uterine M-CSF concentration is regulated by a synergistic action of estradiol and progesterone. M-CSF is produced by uterine glandular epithelial cells. It had been found that FMS, the M-CSF receptor, is expressed in placenta and choriocarcinoma cell lines4.
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, tissue homogenates, serum and plasma (EDTA)
• Sensitivity: <1 pg/ml
• Predicted Homology Based on Immunogen Sequence: No detectable cross-reactivity with any other cytokine
• Reference: 1. Ladner, M. B.; Martin, G. A.; Noble, J. A.; Nikoloff, D. M.; Tal, R.; Kawasaki, E. S.; White, T. J. :
  Human CSF-1: gene structure and alternative splicing of mRNA precursors. EMBO J. 6: 2693-2698, 1987.
  2. Kawasaki, E. S.; Ladner, M. B.; Wang, A. M.; Van Arsdell, J.; Warren, M. K.; Coyne, M. Y.; Schweickart, V. L.; Lee,
  M.-T.; Wilson, K. J.; Boosman, A.; Stanley, E. R.; Ralph, P.; Mark, D. F. : Molecular cloning of a complementary
  DNA encoding human macrophage-specific colony-stimulating factor (CSF-1). Science 230: 291-296, 1985.
  3. Ladner, M. B.; Martin, G. A.; Noble, J. A.; Nikoloff, D. M.; Tal, R.; Kawasaki, E. S.; White, T. J. : Human CSF-1: gene
  structure and alternative splicing of mRNA precursors. EMBO J. 6: 2693-2698, 1987.
  4. Pollard, J. W.; Bartocci, A.; Arceci, R.; Orlofsky, A.; Ladner, M. B.; Stanley, E. R. : Apparent role of the macrophage
  growth factor, CSF-1, in placental development. Nature 330: 484-486, 1987.
• Specificity: Natural and recombinant mouse M-CSF
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: K33-E262
• Description: For quantitative detection of mouse M-CSF in cell culture supernatants, serum and plasma (EDTA).

Target Info

• Gene Symbol: CSF1
• Gene Full Name: colony stimulating factor 1
• Alias Symbols: Colony stimulating factor 1, CSF-1, CSF1, Lanimostim, MCSF, P09603, CSF 1, M CSF, M-CSF
• NCBI Gene Id: 12977
• Protein Name: Macrophage colony-stimulating factor 1
• Description of Target: SF, also called CSF1, consists of a 14-amino acid peptide, longer than the usual 8-to-11 mer recognized by most CTLs. The M-CSF gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb. Although it is a single-copy gene, its expression results in the synthesis of several mRNAs, ranging in size from about 1.5 to 4.5 kb. There are 2 forms of M-CSF, with 224 and 522 amino acids, resulting from alternative splicing. Furthermore, M-CSF has a role in development of the placenta. Uterine M-CSF concentration is regulated by a synergistic action of estradiol and progesterone. M-CSF is produced by uterine glandular epithelial cells. It had been found that FMS, the M-CSF receptor, is expressed in placenta and choriocarcinoma cell lines.
• Uniprot ID: P07141
• Protein Accession #: NP_001107001.1
• Nucleotide Accession #: NM_001113529.1