Catalog No: OKEH04300
Size:96T
Price: $525.00
SKU
OKEH04300
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology GRIN1 ELISA Kit (Human) (OKEH04300) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GRIN1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GRIN1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GRIN1 captured in the well. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: <= 4.7% (n = 20) Mean Inter-assay CV%: <= 9.8% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids. | ||||||||||||||||||||||
Sensitivity | 0.078 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Glutamate receptor ionotropic, NMDA 1 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | GRIN1 |
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Gene Full Name | glutamate ionotropic receptor NMDA type subunit 1 |
Alias Symbols | GluN1;glutamate [NMDA] receptor subunit zeta-1;glutamate receptor ionotropic, NMDA 1;glutamate receptor, ionotropic, N-methyl D-aspartate 1;MRD8;NDHMSD;NDHMSR;NMDA1;NMDAR1;NMD-R1;N-methyl-D-aspartate receptor channel, subunit zeta-1;N-methyl-D-aspartate receptor subunit NR1;NR1;putative NMDtranscript(altAcc_e2). |
NCBI Gene Id | 2902 |
Protein Name | Q05586 |
Description of Target | Glutamate receptor ionotropic, NMDA 1 |
Uniprot ID | https://www.uniprot.org/uniprot/Q05586 |
Protein Accession # | NP_000823.4 |
Nucleotide Accession # | NM_000832.6 |
Protein Size (# AA) | 938 |
Molecular Weight | 105 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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