GRIN1 Antibody (Phospho-Ser890) (OAAF00129)

Catalog No: OAAF00129 (Formerly GWB-ASB281)

Size:100 ug

Click here to learn more about Aviva's By-Request Conjugation Service.

Immunohistochemistry analysis of paraffin-embedded human brain, using NMDAR1 (Phospho-Ser890) Antibody. The picture on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of A549 cells, using NMDAR1 (Phospho-Ser890) Antibody. The picture on the right is blocked with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Paraffin
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human NMDAR1 around the phosphorylation site of Ser890.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Specificity: GRIN1 (Phospho-Ser890) antibody detects endogenous levels of GRIN1 only when phosphorylated at serine 890.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:40000
• Reference: Guang Bai, J. Biol. Chem., Jan 1998; 273: 1086.
  Guang Bai, J. Biol. Chem., Mar 1995; 270: 7737.
  Mai T. Dang, PNAS, Oct 2006; 103: 15254 - 15259.

Target Info

• Gene Symbol: GRIN1
• Gene Full Name: glutamate ionotropic receptor NMDA type subunit 1
• Alias Symbols: GluN1;glutamate [NMDA] receptor subunit zeta-1;glutamate receptor ionotropic, NMDA 1;glutamate receptor, ionotropic, N-methyl D-aspartate 1;MRD8;NDHMSD;NDHMSR;NMDA1;NMDAR1;NMD-R1;N-methyl-D-aspartate receptor channel, subunit zeta-1;N-methyl-D-aspartate receptor subunit NR1;NR1;putative NMDtranscript(altAcc_e2).
• NCBI Gene Id: 2902
• Protein Name: Glutamate receptor ionotropic, NMDA 1
• Description of Target: Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:7685113, PubMed:28126851, PubMed:26919761, PubMed:26875626, PubMed:28105280). Sensitivity to glutamate and channel kinetics depend on the subunit composition (PubMed:26919761).
• Uniprot ID: Q05586
• Protein Accession #: NP_000823.4
• Molecular Weight: 105 kDa