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Datasheets/Manuals | Printable datasheet for Goat Anti-Pericentrin 1 / NUP85 Antibody (OAEB01821) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Clonality | Polyclonal |
Host | Goat |
Application | P-WB, IP |
Additional Information | Important Note: Please note that, in contrast to other anti-Pericentrin antibodies available, this Ab is NOT expected to recognize Kendrin (also known as Pericentrin 2). This may explain why it recognizes a 75KDa band (the expected size according to NP_079120) and not a 220kDa band (as reported for other anti-pericentrin antibodies). |
Reconstitution and Storage | Aliquot and store at -20C. Minimize freezing and thawing. |
Immunogen | NUP85 |
Purification | Ammonium Sulfate Precipitated; Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Human, Mouse, Rat |
Peptide Sequence | C-ARAIIREGSLEGS |
Concentration | 0.5 mg/ml |
Application Info | Peptide ELISA: antibody detection limit dilution 1:32,000 WB: Approx 75kDa band observed in HeLa lysates (calculated MW of 75kDa according to NP_079120). Recommended concentration: 1-3 ug/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on Human: Toda E et al, J Immunol. 2009 Nov 15;183(10):6387-94. PMID: 19841162. Immunoprecipitation: This antibody was successfully used in IP on Human: Toda E et al, J Immunol. 2009 Nov 15;183(10):6387-94. PMID: 19841162. |
Reference | This antibody has been successfully used in WB and IP on Human: Toda E, Terashima Y, Sato T, Hirose K, Kanegasaki S, Matsushima K. FROUNT is a common regulator of CCR2 and CCR5 signaling to control directional migration.J Immunol. 2009 Nov 15;183(10):6387-94. PMID: 19841162 |
Gene Symbol | NUP85|Pcnt |
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Gene Full Name | nucleoporin 85|pericentrin (kendrin) |
Alias Symbols | AW476095;C86676;FROUNT;KEN;kendrin;m239;m239Asp;m275;m275Asp;NPHS17;nuclear pore complex protein Nup85;nucleoporin 85kDa;nucleoporin Nup75;nucleoporin Nup85;Nup75;Pcn;Pcnt2;pericentrin;pericentrin 2;pericentrin-250;pericentrin-360. |
NCBI Gene Id | 18541|79902 |
Protein Name | Nuclear pore complex protein Nup85 |
Uniprot ID | Q9BW27 |
Protein Accession # | NP_079120.1 |
Molecular Weight | 75 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Goat Anti-Pericentrin 1 / NUP85 Antibody (OAEB01821)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "Goat Anti-Pericentrin 1 / NUP85 Antibody (OAEB01821)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "Goat Anti-Pericentrin 1 / NUP85 Antibody (OAEB01821)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Goat Anti-Pericentrin 1 / NUP85 Antibody (OAEB01821)"?
This target may also be called "AW476095;C86676;FROUNT;KEN;kendrin;m239;m239Asp;m275;m275Asp;NPHS17;nuclear pore complex protein Nup85;nucleoporin 85kDa;nucleoporin Nup75;nucleoporin Nup85;Nup75;Pcn;Pcnt2;pericentrin;pericentrin 2;pericentrin-250;pericentrin-360." in publications.
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What is the shipping cost for "Goat Anti-Pericentrin 1 / NUP85 Antibody (OAEB01821)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Goat Anti-Pericentrin 1 / NUP85 Antibody (OAEB01821)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Goat Anti-Pericentrin 1 / NUP85 Antibody (OAEB01821)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "75".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Goat Anti-Pericentrin 1 / NUP85 Antibody (OAEB01821)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "NUP85|Pcnt"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "NUP85|Pcnt"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "NUP85|Pcnt"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "NUP85|Pcnt"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "NUP85|Pcnt"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "NUP85|Pcnt"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.