GHRH Antibody - N-terminal region (OAAB04805)

Catalog No: OAAB04805

Size:400ul

GHRH Antibody (N-term) (Cat.#OAAB04805) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the GHRH antibody detected the GHRH protein (arrow).

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GHRH antibody (N-term) (Cat.#OAAB04805), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of GHRH Antibody (N-term) (Cat. #OAAB04805) with 293 cell followed by Alexa Fluor®488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC-P, IF
  Function: GRF is released by the hypothalamus and acts on the adenohypophyse to stimulate the secretion of growth hormone.
  Cellular Location: Secreted
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This GHRH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-34 amino acids from the N-terminal region of human GHRH.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  GHRH is strongly supported by BioGPS gene expression data to be expressed in MDA-MB231

• Application Info: WB: 1:1000
  IF: 1:10~50
  IHC-P: 1:10~50

Target Info

• Gene Symbol: GHRH
• Alias Symbols: GRF, INN, GHRF
• NCBI Gene Id: 2691
• Protein Name: Somatoliberin
• Description of Target: GHRH belongs to the glucagon family and is a preproprotein that is produced in the hypothalamus. The preproprotein is cleaved to form a 44 aa factor, also called somatocrinin, that acts to stimulate growth hormone release from the pituitary. Variant receptors for somatocrinin have been found in several types of tumors, and antagonists of these receptors can inhibit the growth of the tumors. Defects in this protein are a cause of dwarfism, while hypersecretion of the encoded protein is a cause of gigantism.
• Uniprot ID: P01286
• Protein Accession #: NP_001171660.1
• Molecular Weight: 12447 Da