CDC25A ELISA Kit (Human) (OKCD00442)

Catalog No: OKCD00442 (Formerly GWB-KBBFI3)

Size:96WELLS

Human CDC25A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	82-97%	80-92%	78-93%	81-89%
  EDTA plasma(n=5)	96-103%	91-99%	80-90%	89-97%
  heparin plasma(n=5)	94-102%	99-105%	90-102%	92-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cell Division Cycle Protein 25 (CDC25). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cell Division Cycle Protein 25 (CDC25). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cell Division Cycle Protein 25 (CDC25), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cell Division Cycle Protein 25 (CDC25) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	79-97	88
  EDTA plasma(n=5)	85-104	88
  heparin plasma(n=5)	80-101	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cell Division Cycle Protein 25 (CDC25) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cell Division Cycle Protein 25 (CDC25) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CDC25A Microplate: 96 Wells (12 x 8 Well Strips)
  • CDC25A Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated CDC25A Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.063 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cell Division Cycle Protein 25 (CDC25). No significant cross-reactivity or interference between Cell Division Cycle Protein 25 (CDC25) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CDC25A
• Gene Full Name: cell division cycle 25A
• Alias Symbols: CDC25 homolog A;CDC25A2;dual specificity phosphatase CDC25A;M-phase inducer phosphatase 1.
• NCBI Gene Id: 993
• Protein Name: M-phase inducer phosphatase 1
• Description of Target: Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
• Uniprot ID: P30304
• Protein Accession #: NP_001780
• Nucleotide Accession #: NM_001789.2
• Protein Size (# AA): 524